In vivo nuclear delivery of oligonucleotides via hybridizing bifunctional peptides

被引：48

作者：

Brandén, LJ ^{[1
]}

Christensson, B

Smith, CIE

机构：

[1] Karolinska Inst, Novum, Dept Biosci, SE-14157 Huddinge, Sweden

[2] Huddinge Univ Hosp, Karolinska Inst, Clin Res Ctr, S-14186 Huddinge, Sweden

[3] Huddinge Univ Hosp, Dept Immunol Microbiol Pathol & Infect Dis, S-14186 Huddinge, Sweden

来源：

GENE THERAPY | 2001年 / 8卷 / 01期

关键词：

PNA; NLS; in vivo; transfection; gene therapy;

D O I：

10.1038/sj.gt.3301345

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Linking proteins directly to nucleic acids has been a complex task. By hybridizing a bifunctional peptide nucleic acid (PNA) consisting of a nucleic acid binding moiety and a nuclear localization signal (NLS) we have previously demonstrated that it is possible to link protein functions directly to nucleic acids containing a PNA target site. By hybridizing fluorescently labeled oligonucleotides to PNA-NLS molecules and subsequently transfecting different organs in vivo we demonstrate an active nuclear translocation of the PNA-NLS/oligonucleotide complex in different mouse organs.

引用

页码：84 / 87

页数：4

共 9 条

[1] A VERSATILE VECTOR FOR GENE AND OLIGONUCLEOTIDE TRANSFER INTO CELLS IN CULTURE AND IN-VIVO - POLYETHYLENIMINE
BOUSSIF, O
LEZOUALCH, F
ZANTA, MA
MERGNY, MD
SCHERMAN, D
DEMENEIX, B
BEHR, JP
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (16) : 7297 - 7301
[2] A peptide nucleic acid-nuclear localization signal fusion that mediates nuclear transport of DNA
Brandén, LJ
Mohamed, AJ
Smith, CIE
[J]. NATURE BIOTECHNOLOGY, 1999, 17 (08) : 784 - 787
[3] Coupling of cell-binding ligands to polyethylenimine for targeted gene delivery
Kircheis, R
Kichler, A
Wallner, G
Kursa, M
Ogris, M
Felzmann, T
Buchberger, M
Wagner, E
[J]. GENE THERAPY, 1997, 4 (05) : 409 - 418
[4] Cloning of two novel human importin-alpha subunits and analysis of the expression pattern of the importin-alpha protein family
Kohler, M
Ansieau, S
Prehn, S
Leutz, A
Haller, H
Hartmann, E
[J]. FEBS LETTERS, 1997, 417 (01): : 104 - 108
[5] Hydrodynamics-based transfection in animals by systemic administration of plasmid DNA
Liu, F
Song, YK
Liu, D
[J]. GENE THERAPY, 1999, 6 (07) : 1258 - 1266
[6] Differential modes of nuclear localization signal (NLS) recognition by three distinct classes of NLS receptors
Miyamoto, Y
Imamoto, N
Sekimoto, T
Tachibana, T
Seki, T
Tada, S
Enomoto, T
Yoneda, Y
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (42) : 26375 - 26381
[7] CYANINE DYE LABELING REAGENTS - SULFOINDOCYANINE SUCCINIMIDYL ESTERS
MUJUMDAR, RB
ERNST, LA
MUJUMDAR, SR
LEWIS, CJ
WAGGONER, AS
[J]. BIOCONJUGATE CHEMISTRY, 1993, 4 (02) : 105 - 111
[8] Nielsen PE, 2000, PHARMACOL TOXICOL, V86, P3
[9] A LONG SYNTHETIC PEPTIDE CONTAINING A NUCLEAR-LOCALIZATION SIGNAL AND ITS FLANKING SEQUENCES OF SV40 T-ANTIGEN DIRECTS THE TRANSPORT OF IGM INTO THE NUCLEUS EFFICIENTLY
YONEDA, Y
SEMBA, T
KANEDA, Y
NOBLE, RL
MATSUOKA, Y
KURIHARA, T
OKADA, Y
IMAMOTO, N
[J]. EXPERIMENTAL CELL RESEARCH, 1992, 201 (02) : 313 - 320

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