Expression and characterization of recombinant NH2-terminal cell binding fragment of vitronectin in E-coli

被引：3

作者：

Jang, JH

Koak, JY

Kim, SC

Hwang, JH

Lee, JB

Jang, IT

Chung, CP

Heo, SJ ^{[1
]}

机构：

[1] Seoul Natl Univ, Coll Dent, Dept Prosthodont, Seoul 110768, South Korea

[2] Seoul Natl Univ, Coll Dent, Intellectual Biointerface Engn Ctr, Seoul 110768, South Korea

来源：

BIOTECHNOLOGY LETTERS | 2003年 / 25卷 / 23期

关键词：

cell adhesion; extracellular matrix; peptide; vitronectin; ADHESION; IDENTIFICATION; PROTEIN; DOMAIN;

D O I：

10.1023/B:BILE.0000004387.96353.21

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A recombinant peptide fragment of vitronectin (rVN(143)), that includes the Arg-Gly-Asp (RGD) cell recognition site, was expressed in Escherichia coli using a prokaryotic expression system. The addition of recombinant rVN(143) peptide enhances cell adhesion and proliferation similar (similar to 70%) to those of native VN.

引用

页码：1973 / 1975

页数：3

共 7 条

[1]

BALE MD, 1989, BLOOD, V74, P2698

[2] REQUIREMENT OF VASCULAR INTEGRIN ALPHA(V)BETA(3) FOR ANGIOGENESIS [J].