Antitumor activity of Pulsatilla chinensis (Bunge) Regel saponins in human liver tumor 7402 cells in vitro and in vivo

被引：101

作者：

Xu, Qiong-ming ^{[1
]}

Shu, Zhan ^{[1
]}

He, Wen-jun ^{[1
]}

Chen, Lan-ying ^{[2
]}

Yang, Shi-lin ^{[1
,2
]}

Yang, Gang ^{[3
]}

Liu, Yan-li ^{[1
]}

Li, Xiao-ran ^{[1
]}

机构：

[1] Soochow Univ, Coll Pharm, Suzhou 215123, Peoples R China

[2] Jiangxi Univ Tradit Chinese Med, Nanchang 330004, Peoples R China

[3] Soochow Univ, Electron Microscope Lab Expt Ctr, Suzhou 215123, Peoples R China

来源：

PHYTOMEDICINE | 2012年 / 19卷 / 3-4期

关键词：

Pulsatilla chinensis (Bunge) Regel; Saponins; Human liver tumor 7402 cells; Antitumor; TRITERPENOID SAPONINS; GLYCOSIDES; ROOTS;

D O I：

10.1016/j.phymed.2011.08.066

中图分类号：

Q94 [植物学];

学科分类号：

071001 [植物学];

摘要：

Pulsatilla chinensis (Bunge) Regel is a Chinese medicinal herb for "blood-cooling" and detoxification. Now it is used for the treatment of malignant tumor, but the antitumor mechanisms and toxic side effects of P. chinensis are unclear. The present study was undertaken to investigate if P. chinensis saponins (PRS) possesses anticancer effects and toxic side effects in human liver tumor 7402 cells in vitro and vivo. 7402 cells were treated with different concentrations of PRS for 24 h. Cell viability was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Cell apoptosis was assessed by flow cytometry. The in vivo effect of PRS on 7402 tumor cells transplanted in athymic nude mice was investigated. 15 saponins were isolated and identified from PRS. PRS inhibited the proliferation of human liver tumor 7402 cells in vitro by apoptosis. 19 days after administration of PRS (100, 200 mg/kg), the weight of tumor mass was markly decreased in nude mice. The anti-tumor effect of PRS in vivo was associated with a significant increase in the 7402 apoptosis rate. Although PRS inhibited the weight of mice, it showed almost no effect on leukocyte number, liver and spleen weight index. Light microscopic histopathological examination showed that PRS had no specific lesion in organ. These results suggested that P. chinensis saponins exert potential anticancer activity in treating tumors in nude mice and no toxic side effects. (C) 2011 Elsevier GmbH. All rights reserved.

引用

页码：293 / 300

页数：8

共 24 条

[21]

23-Hydroxybetulinic acid from Pulsatilla chinensis (Bunge) Regel synergizes the antitumor activities of doxorubicin in vitro and in vivo [J].