The carboxyl-terminal region of Crtac1B/LOTUS acts as a functional domain in endogenous antagonism to Nogo receptor-1

被引：22

作者：

Kurihara, Yuji ^{[1
,2
]}

Arie, Yuko ^{[1
,2
]}

Iketani, Masumi ^{[1
]}

Ito, Hiromu ^{[1
]}

Nishiyama, Kuniyuki ^{[1
]}

Sato, Yasufumi ^{[1
]}

Nakamura, Fumio ^{[1
]}

Mizuki, Nobuhisa ^{[2
]}

Goshima, Yoshio ^{[1
,3
]}

Takei, Kohtaro ^{[1
,3
]}

机构：

[1] Yokohama City Univ, Grad Sch Med, Kanazawa Ward, Dept Mol Pharmacol & Neurobiol, Yokohama, Kanagawa 2360004, Japan

[2] Yokohama City Univ, Grad Sch Med, Kanazawa Ward, Dept Ophthalmol, Yokohama, Kanagawa 2360004, Japan

[3] Yokohama City Univ, Grad Sch Med, Kanazawa Ward, Adv Med Res Ctr, Yokohama, Kanagawa 2360004, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2012年 / 418卷 / 02期

关键词：

Crtac1B; LOTUS; Nogo receptor-1; Functional domain; Antagonism; OLIGODENDROCYTE-MYELIN GLYCOPROTEIN; SPINAL-CORD-INJURY; AXON REGENERATION; GROWTH; RECOVERY; INHIBITION; PROTEIN; IDENTIFICATION; CARTILAGE; MODULES;

D O I：

10.1016/j.bbrc.2012.01.033

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Myelin-derived axon growth inhibitors, such as Nog, bind to Nogo receptor-1 (NgR1) and thereby limit the action of axonal regeneration after injury in the adult central nervous system. Recently, we have found that cartilage acidic protein-1B (Crtac1B)/lateral olfactory tract usher substance (LOTUS) binds to NgR1 and functions as an endogenous NgR1 antagonist. To examine the functional domain of LOTUS in the antagonism to NgR1, analysis using the deletion mutants of LOTUS was performed and revealed that the carboxyl-terminal region (UA/EC domain) of LOTUS bound to NgR1. The UA/EC fragment of LOTUS overexpressed together with NgR1 in COS7 cells abolished the binding of Nogo66 to NgR1. Overexpression of the UA/EC fragment in cultured chick dorsal root ganglion neurons suppressed Nogo66-induced growth cone collapse. These findings suggest that the UA/EC region is a functional domain of LOTUS serving for an antagonistic action to NgR1. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：390 / 395

页数：6

共 26 条

[1] MAG and OMgp Synergize with Nogo-A to Restrict Axonal Growth and Neurological Recovery after Spinal Cord Trauma [J].