Nonswelling macroporous synbeads for improved efficiency of solid-phase biotransformations

被引:19
作者
Basso, A
Braiuca, P
De Martin, L
Ebert, C
Gardossi, L
Linda, P
Verdelli, S
Tam, A
机构
[1] Univ Trieste, Dipartimento Sci Farmaceut, I-34127 Trieste, Italy
[2] Mitsubishi Chem Corp, Resindion SrL, I-20082 Milan, Italy
关键词
biotransformations; combinatorial chemistry; enzyme catalysis; penicillin G acylase; polymer resins; solid-phase synthesis;
D O I
10.1002/chem.200305243
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
An application of novel, highly porous nonswelling resins (Synbeads) for enzymatic catalysis on solid supports is reported. These new resins combine easy handling of the beads, chemical stability, improved accessibility of proteins and higher productivity relative to swelling polymers. The present study demonstrates that the resin porosity greatly affects the efficiency in solid-phase biotransformations and that Synbead resins are valuable alternatives to swelling polymers for solid-phase chemistry and biocatalysis. The present study investigates the influence of key parameters, such as porosity and reactive functional-group density, on the reaction efficiency.
引用
收藏
页码:1007 / 1013
页数:7
相关论文
共 41 条
[1]   Combinatorial biocatalysis: taking the lead from Nature [J].
Altreuter, DH ;
Clark, DS .
CURRENT OPINION IN BIOTECHNOLOGY, 1999, 10 (02) :130-136
[2]  
Balkenhohl F, 1996, ANGEW CHEM INT EDIT, V35, P2289
[3]  
Balkenhohl F., 1996, ANGEW CHEM, V108, P2436
[4]   Organically modified xerogels as supports for solid-phase chemistry [J].
Basso, A ;
De Martin, L ;
Ebert, C ;
Linda, P ;
Gardossi, L ;
Ulijn, RV ;
Flitsch, SL .
TETRAHEDRON LETTERS, 2003, 44 (32) :6083-6085
[5]   Improved biotransformations on charged PEGA supports [J].
Basso, A ;
De Martin, L ;
Gardossi, L ;
Margetts, G ;
Brazendale, I ;
Bosma, AY ;
Ulijn, RV ;
Flitsch, SL .
CHEMICAL COMMUNICATIONS, 2003, (11) :1296-1297
[6]  
Bergbreiter DE, 1999, MED RES REV, V19, P439, DOI 10.1002/(SICI)1098-1128(199909)19:5<439::AID-MED9>3.0.CO
[7]  
2-7
[8]  
Fruchtel J. S., 1996, ANGEW CHEM, V108, P19
[9]   Organic chemistry on solid supports [J].
Fruchtel, JS ;
Jung, G .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH, 1996, 35 (01) :17-42
[10]  
Grether U, 2001, CHEM-EUR J, V7, P959, DOI 10.1002/1521-3765(20010302)7:5<959::AID-CHEM959>3.3.CO