Human T lymphocytes express N-methyl-D-aspartate receptors functionally active in controlling T cell activation

被引：79

作者：

Miglio, G ^{[1
]}

Varsaldi, F ^{[1
]}

Lombardi, G ^{[1
]}

机构：

[1] Eastern Piedmont Univ, DiSCAFF Dept, I-28100 Novara, Italy

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2005年 / 338卷 / 04期

关键词：

glutamate receptors; NMDA receptors; NMDA receptor antagonists; human lymphocytes; T cell proliferation; T cell activation; cell cycle;

D O I：

10.1016/j.bbrc.2005.10.164

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of this study was to investigate the expression and the functional role of N-methyl-D-aspartate (NMDA) receptors in human T cells. RT-PCR analysis showed that human resting peripheral blood lymphocytes (PBL) and Jurkat T cells express genes encoding for both NR1 and NR2B subunits: phytohemagglutinin (PHA)-activated PBL also expresses both these genes and the NR2A and NR2D genes. Cytofluorimetric analysis showed that NR1 expression increases as a consequence of PHA (10 pg/ml) treatment. D-(-)-2-Amino-5-phosphonopentanoic acid (D-AP5), and (+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5,10-imine [(+)-MK 801], competitive and non-competitive NMDA receptor antagonists, respectively, inhibited PHA-induced T cell proliferation, whereas they did not affect IL-2 (10 U/ml)-induced proliferation of PHA blasts. These effects were due to the prevention of T cell activation (inhibition of cell aggregate formation and CD25 expression), but not to cell cycle arrest or death. These results demonstrate that human T lymphocytes express NMDA receptors, which are functionally active in controlling cell activation. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：1875 / 1883

页数：9