Instrumental setup for high-throughput small- and wide-angle solution scattering at the X33 beamline of EMBL Hamburg

被引:63
作者
Blanchet, Clement E. [1 ]
Zozulya, Alexey V. [1 ]
Kikhney, Alexey G. [1 ]
Franke, Daniel [1 ]
Konarev, Peter V. [1 ]
Shang, Weifeng [1 ]
Klaering, Robbert [1 ]
Robrahn, Bernd [1 ]
Hermes, Christoph [1 ]
Cipriani, Florent [2 ]
Svergun, Dmitri I. [1 ]
Roessle, Manfred [1 ]
机构
[1] EMBL Hamburg, D-22603 Hamburg, Germany
[2] EMBL Grenoble, F-38042 Grenoble, France
关键词
SYNCHROTRON-RADIATION; CONTROL-SYSTEM; DIFFRACTION; DETECTOR;
D O I
10.1107/S0021889812013490
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A setup is presented for automated high-throughput measurements of small-angle X-ray scattering (SAXS) from macromolecular solutions on the bending-magnet beamline X33 of EMBL at the storage ring DORIS-III (DESY, Hamburg). A new multi-cell compartment allows for rapid switching between in-vacuum and in-air operation, for digital camera assisted control of cell filling and for colour sample illumination. The beamline is equipped with a Pilatus 1 M-W pixel detector for SAXS and a Pilatus 300 k-W for wide-angle scattering (WAXS), and results from the use of the Pilatus detectors for scattering studies are reported. The setup provides a broad resolution range from 100 to 0.36 nm without the necessity of changing the sample-to-detector distance. A new optimized robotic sample changer is installed, permitting rapid and reliable automated sample loading and cell cleaning with a required sample volume of 40 ml. All the devices are fully integrated into the beamline control software system, ensuring fully automated and user-friendly operation (attended, unattended and remote) with a throughput of up to 15 measurements per hour.
引用
收藏
页码:489 / 495
页数:7
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