Genetic differences in nitrate uptake in two clones of the common reed, Phragmites australis

被引:9
作者
Araki, R
Mori, M
Mori, M
Hasegawa, H
机构
[1] Univ Shiga Prefecture, Grad Sch Environm Sci, Shiga 5228533, Japan
[2] Shiga Prefecture Agr Technol Promot Ctr, Shiga 5211301, Japan
[3] Ishikawa Prefectural Univ, Res Inst Bioresources & Biotechnol, Nonoichi, Ishikawa 9218836, Japan
关键词
Phragmites australis; high-affinity nitrate transporter; nitrate uptake; Michaelis-Menten kinetics; gene isolation;
D O I
10.1270/jsbbs.55.297
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Phragmites australis, common reed, could be useful in removing eutrophic substances from river and lake water. In this study, the genetic differences in nitrate uptake ability of the reed were investigated with a view to breeding a reed plant useful for phytoremediation. Two reed clones (W-6 and W-8) isolated from a reed community in the lakeside wetland along Lake Biwa, Japan, were used for a study on the physiological and molecular basis of nitrate uptake. K(m)s for nitrate uptake were 80.8 and 45.2 mu M and V(max)s for nitrate uptake were 10.62 and 2.37 mu mol g(-1) root f.w. h(-1) in W-6 and W-8, respectively, suggesting that there were critical differences in kinetic parameters for nitrate uptake. To investigate these differences at the molecular level, we isolated a high-affinity nitrate transporter gene (NRT2) from the two reed clones and analyzed the reed NRT2 structure and expression. The deduced amino acid sequence of the reed NRT2 consisted of 523 residues and had a high similarity to NRT2 from other monocots. Reed NRT2 was strongly expressed in roots treated with 200 mu M nitrate. There were three amino acid substitutions of the reed NRT2 between W-6 and W-8. Differences in NRT2 transcription were also observed between the two clones. It was not clear whether the difference in kinetic parameters for nitrate was due to the reed NRT2 structure or expression. These results indicate the possibility of selecting genotypes more useful for the removal of nitrate.
引用
收藏
页码:297 / 302
页数:6
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