LeNRT2.3 functions in nitrate acquisition and long-distance transport in tomato

被引：45

作者：

Fu, Yanlei ^{[1
]}

Yi, Hongying ^{[1
]}

Bao, Juan ^{[1
]}

Gong, Jiming ^{[1
]}

机构：

[1] Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Natl Key Lab Plant Mol Genet, Shanghai 200032, Peoples R China

来源：

FEBS LETTERS | 2015年 / 589卷 / 10期

基金：

美国国家科学基金会;

关键词：

Tomato; LeNRT2.3; Nitrate; Acquisition; Long-distance transport; Low-affinity; ARABIDOPSIS NITRATE; CHLAMYDOMONAS-REINHARDTII; AMMONIUM TRANSPORTER; UPTAKE SYSTEM; PLANTS; EXPRESSION; ROOTS; GENE; NITROGEN; MUTATION;

D O I：

10.1016/j.febslet.2015.03.016

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nitrogen plays an important role in plant growth and development. Nitrate transporters have been extensively studied in Arabidopsis, but in tomato they have not been functionally characterized. In this study, we report the functions of LeNRT2.3 in nitrate transport in tomato. Our results show that LeNRT2.3 is induced by nitrate, and mainly localizes to the plasma membranes of rhizodermal and pericycle cells in roots. Further analysis in Xenopus oocytes showed that LeNRT2.3 mediates low-affinity nitrate transport. 35S: LeNRT2.3 increased nitrate uptake in root and transport from root to shoot. More interestingly, 35S: LeNRT2.3 showed high biomass and fruit weight. Taken together, these results suggest that LeNRT2.3 plays a double role in nitrate uptake and long-distance transport in tomato. (C) 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：1072 / 1079

页数：8

共 55 条

[1] Expression of rice (Oryza sativa L.) genes involved in high-affinity nitrate transport during the period of nitrate induction [J].