Coherent anti-Stokes Raman scattering hyperspectral tissue imaging with a wavelength-swept system

被引：84

作者：

Begin, Steve ^{[1
,2
]}

Burgoyne, Bryan ^{[3
]}

Mercier, Vincent ^{[3
]}

Villeneuve, Alain ^{[3
]}

Vallee, Real ^{[2
]}

Cote, Daniel ^{[1
,2
]}

机构：

[1] Univ Laval, CRULRG, Quebec City, PQ G1J 2G3, Canada

[2] Univ Laval, COPL, Quebec City, PQ G1V 0A6, Canada

[3] Genia Photon Inc, La Salle, PQ H8N 2J4, Canada

来源：

BIOMEDICAL OPTICS EXPRESS | 2011年 / 2卷 / 05期

关键词：

HIGH-SENSITIVITY; CARS MICROSCOPY; LASER;

D O I：

10.1364/BOE.2.001296

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

We present a wavelength-swept coherent anti-Stokes Raman scattering (WS-CARS) spectroscopy system for hyperspectral imaging in thick tissue. We use a strategy where the Raman lines are excited sequentially, circumventing the need for a spectrometer. This fibre laser system, consisting of a pump laser synchronized with a rapidly tunable programmable laser (PL), can access Raman lines over a significant fraction of the high wavenumber region (2700-2950 cm-1) at rates of up to 10,000 spectral points per second. To demonstrate its capabilities, we have acquired WS-CARS spectra of several samples as well as images and hyperspectral images (HSI) of thick tissue both in forward and epi-detection. This instrument should be especially useful in providing local biochemical information with surrounding context supplied by imaging. (C) 2011 Optical Society of America

引用

页码：1296 / 1306

页数：11

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