Multiplex coherent anti-stokes Raman scattering microspectroscopy and study of lipid vesicles

被引：308

作者：

Cheng, JX ^{[1
]}

Volkmer, A ^{[1
]}

Book, LD ^{[1
]}

Xie, XS ^{[1
]}

机构：

[1] Harvard Univ, Dept Chem & Biol Chem, Cambridge, MA 02138 USA

来源：

JOURNAL OF PHYSICAL CHEMISTRY B | 2002年 / 106卷 / 34期

关键词：

D O I：

10.1021/jp025771z

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

We report a theoretical description and experimental implementation of multiplex coherent anti-Stokes Raman scattering (M-CARS) microspectroscopy using a picosecond pump beam and a femtosecond Stokes beam. The effect of the chirp in the Stokes pulse on a M-CARS spectrum is studied. Polarization-sensitive detection is utilized for suppression of the nonresonant background and for selective detection of spectrally overlapped Raman bands. M-CARS spectra of liposomes in the C-H stretching vibration region were acquired. Polarization CARS imaging of lipids in live cells based on the M-CARS spectra is demonstrated.

引用

页码：8493 / 8498

页数：6

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