A V-shaped cationic dye for nonlinear optical bioimaging

被引:58
作者
Macoas, Ermelinda [1 ,2 ]
Marcelo, Gema [1 ,2 ]
Pinto, Sandra [1 ,2 ]
Caneque, Tatiana [3 ]
Cuadro, Ana M. [3 ]
Vaquero, Juan J. [3 ]
Martinho, Jose M. G. [1 ,2 ]
机构
[1] Inst Super Tecn, CQFM, P-1049001 Lisbon, Portugal
[2] Inst Super Tecn, Inst Nanosci & Nanotechnol IN, P-1049001 Lisbon, Portugal
[3] Univ Alcala, Dept Quim Organ, Madrid 28871, Spain
关键词
2-PHOTON ABSORPTION CHROMOPHORES; STYRYL DYES; DNA; EXCITATION; DESIGN;
D O I
10.1039/c1cc12163d
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A symmetric cationic molecule with D-pi-A(+)-pi-D architecture was synthesized with high two-photon absorption cross-section (sigma(2) approximate to 1140 GM). Application as a marker in fluorescence microscopy of living cells revealed its presence inside the cell staining vesicular shape organelles in the cytoplasm. Fluorescence lifetime imaging microscopy shows that it is also able to penetrate within the nucleus.
引用
收藏
页码:7374 / 7376
页数:3
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