Modulation of HIV-like particle assembly in vitro by inositol phosphates

被引：172

作者：

Campbell, S ^{[1
]}

Fisher, RJ

Towler, EM

Fox, S

Issaq, HJ

Wolfe, T

Phillips, LR

Rein, A

机构：

[1] NCI, HIV Drug Resistance Program, Frederick, MD 21702 USA

[2] NCI, Sci Applicat Int Corp, Frederick, MD 21702 USA

[3] NCI, Dev Therapeut Program, Frederick, MD 21702 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2001年 / 98卷 / 19期

关键词：

retrovirus assembly; phosphatidylinsitol phosphates;

D O I：

10.1073/pnas.191224698

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

HIV-1 Gag protein assembles into 100- to 120-nm diameter particles in mammalian cells. Recombinant HIV-1 Gag protein assembles in a fully defined system in vitro into particles that are only 25-30 nm in diameter and that differ significantly in other respects from authentic particles. However, particles with the size and other properties of authentic virions were obtained in vitro by addition of inositol phosphates or phosphatidylinsitol phosphates to the assembly system. Thus, the interactions between HIV-1 Gag protein molecules are altered by binding of inositol derivatives; this binding is apparently essential for normal HIV-1 particle assembly. This requirement is not seen in a deleted Gag protein lacking residues 16-99 within the matrix domain.

引用

页码：10875 / 10879

页数：5

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