Application of a chimeric synthetic peptide in the development of a serologic method for the diagnosis of hepatitis G virus infection

被引：5

作者：

Fernandez-Vidal, M. ^{[1
]}

Cubero, M. D. ^{[1
]}

Ercilla, G. ^{[2
]}

Gomara, M. J. ^{[1
]}

Haro, I. ^{[1
]}

机构：

[1] CSIC, IIQAB, Dept Peptide & Protein Chem, ES-08034 Barcelona, Spain

[2] Hosp Clin Barcelona, ICII, IDIBAPS, Serv Immunol, Barcelona, Spain

来源：

PROTEIN AND PEPTIDE LETTERS | 2007年 / 14卷 / 09期

关键词：

hepatitis G virus/GB virus C; solid-phase peptide synthesis; chimeric peptides; ELISA; circular dichroism; Fourier transform infrared spectroscopy;

D O I：

10.2174/092986607782110239

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

New putative antigenic peptides corresponding to the N- and C-terminal of the E2 envelope protein of GBV-C/HGV were synthesized using solid-phase chemistry. The antigens were obtained in linear and chimeric forms with the main aim of improving the sensitivity of the enzyme immunoassays. Furthermore, CD and FTIR have been used in conjunction to characterize their conformational changes showing that the chimeric peptide presents a more ordered secondary structure than its parent peptides.

引用

页码：865 / 870

页数：6

共 23 条

[1] GB virus C: Insights into co-infection [J].

Berzsenyi, MD ;

Bowden, DS ;

Roberts, SK .

JOURNAL OF CLINICAL VIROLOGY, 2005, 33 (04) :257-266

[2] DETERMINATION OF HELIX AND BETA-FORM OF PROTEINS IN AQUEOUS-SOLUTION BY CIRCULAR-DICHROISM [J].

CHEN, YH ;

YANG, JT ;

CHAU, KH .

BIOCHEMISTRY, 1974, 13 (16) :3350-3359

[3]

Drakopoulou E, 2000, J PEPT SCI, V6, P175

[4] Synthetic peptide-based immunoassay as a supplemental test for HCV infection [J].

El Awady, MK ;

El-Demellawy, MA ;

Khalil, SB ;

Galal, D ;

Goueli, SA .

CLINICA CHIMICA ACTA, 2002, 325 (1-2) :39-46

[5] Assessment of synthetic peptides for hepatitis A diagnosis using biosensor technology [J].

Gómara, MJ ;

Ercilla, G ;

Alsina, MA ;

Haro, I .

JOURNAL OF IMMUNOLOGICAL METHODS, 2000, 246 (1-2) :13-24

[6]

Halasz R, 2001, SCAND J INFECT DIS, V33, P572, DOI 10.1080/00365540110027123

[7] Antigenicity of chimeric synthetic peptides based on HTLV-1 antigens and the impact of epitope orientation [J].

Hernández, M ;

Selles, ME ;

Peña, LP ;

Gómez, I ;

Melchor, A .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2000, 276 (03) :1085-1088

[8] PREDICTION OF PROTEIN ANTIGENIC DETERMINANTS FROM AMINO-ACID-SEQUENCES [J].

HOPP, TP ;

WOODS, KR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (06) :3824-3828

[9] SURFACE AND INSIDE VOLUMES IN GLOBULAR PROTEINS [J].

JANIN, J .

NATURE, 1979, 277 (5696) :491-492

[10] COLOR TEST FOR DETECTION OF FREE TERMINAL AMINO GROUPS IN SOLID-PHASE SYNTHESIS OF PEPTIDES [J].

KAISER, E ;

COLESCOT.RL ;

BOSSINGE.CD ;

COOK, PI .

ANALYTICAL BIOCHEMISTRY, 1970, 34 (02) :595-&

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