Multiplexed particle-based anti-granulocyte macrophage colony stimulating factor assay used as pulmonary diagnostic test

被引：12

作者：

Bonfield, TL

John, N

Barna, BP

Kavuru, MS

Thomassen, MJ

Yen-Lieberman, B

机构：

[1] Cleveland Clin Fdn, Dept Pulm Allergy & Crit Care Med, Cleveland, OH 44195 USA

[2] Cleveland Clin Fdn, Dept Clin Pathol, Cleveland, OH 44195 USA

[3] Cleveland Clin Fdn, Dept Cell Biol, Cleveland, OH 44195 USA

来源：

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY | 2005年 / 12卷 / 07期

关键词：

D O I：

10.1128/CDLI.12.7.821-824.2005

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Pulmonary alveolar proteinosis (PAP) is characterized by the accumulation of lipoproteinaceous material within the lung alveoli. Recent studies indicate that PAP is an autoimmune disease characterized by a neutralizing anti-granulocyte macrophage colony stimulating factor (GM-CSF) antibody. At present the only definitive diagnostic test for PAP is open lung biopsy. We have previously published that anti-GM-CSF is diagnostic for PAP and correlates with disease pathogenesis using a traditional serial anti-GM-CSF antibody titer format (T. L. Bonfield, M. S. Kavuru, and M. J. Thomassen, Clin. Immunol. 105:342-350, 2002). Titer analysis is a semiquantitative method, and often subtle changes in antibody titer are not detectable. In this report we present data to support anti-GM-CSF detection by a quantitative highly sensitive multiplexed particle-based assay which has the potential to be a clinical diagnostic test.

引用

收藏

页码：821 / 824

页数：4

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