Protection of U937 cells from free radical damage by the macrophage synthesized antioxidant 7,8-dihydroneopterin

被引：37

作者：

Gieseg, SP ^{[1
]}

Whybrow, J ^{[1
]}

Glubb, D ^{[1
]}

Rait, C ^{[1
]}

机构：

[1] Univ Canterbury, Dept Zool, Free Rad Biochem Lab, Christchurch 1, New Zealand

来源：

FREE RADICAL RESEARCH | 2001年 / 35卷 / 03期

关键词：

neopterin; macrophage; antioxidant; free-radical; peroxidation; hypochlorite;

D O I：

10.1080/10715760100300841

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Interferon-gamma stimulation of human macrophages causes the synthesis and release of neopterin and its reduced form 7,8-dihydroneopterin (7,8-NP). The purpose of this cellular response is undetermined but in vitro experiments suggests 7,8-NP is an antioxidant. We have found 7,8-NP can protect monocyte-like U937 cells from oxidative damage. 7,8-NP inhibited ferrous ion and hypochlorite mediated loss of cell viability. Fe++ mediated lipid peroxidation was effectively inhibited by 7,8-NP, however no correlation was found between peroxide concentration and cell viability. Hypochlorite was scavenged by 7,8-NP, preventing the loss of cell viability. 7,8-NP was less effective in inhibiting H2O2-mediated loss of cell viability with significant inhibition only occurring at high 7,8-NP concentrations. Analysis of cellular protein hydrolysates showed none of the oxidants caused the formation of any protein bound DOPA or dityrosine but did show 7,8-NP prevented the loss of cellular tyrosine by HOCL Our data suggests macrophages may synthesize 7,8-NP for antioxidant protection during inflammatory events in vivo.

引用

页码：311 / 318

页数：8

共 25 条

[21] FREE-RADICAL AND ENZYMATIC MECHANISMS FOR THE GENERATION OF PROTEIN-BOUND REDUCING MOIETIES [J].

SIMPSON, JA ;

GIESEG, SP ;

DEAN, RT .

BIOCHIMICA ET BIOPHYSICA ACTA, 1993, 1156 (02) :190-196

[22]

VANDENBERG J, 1994, METHOD ENZYMOL, V233, P639

[23] Inhibition of xanthine oxidase by pterins [J].

Wede, I ;

Altindag, ZZ ;

Widner, B ;

Wachter, H ;

Fuchs, D .

FREE RADICAL RESEARCH, 1998, 29 (04) :331-338

[24] NEOPTERIN MODULATES TOXICITY MEDIATED BY REACTIVE OXYGEN AND CHLORIDE SPECIES [J].

WEISS, G ;

FUCHS, D ;

HAUSEN, A ;

REIBNEGGER, G ;

WERNER, ER ;

WERNERFELMAYER, G ;

SEMENITZ, E ;

DIERICH, MP ;

WACHTER, H .

FEBS LETTERS, 1993, 321 (01) :89-92

[25]

WOLFF SP, 1994, METHOD ENZYMOL, V233, P182

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