Identification and functional analysis of a Kluyveromyces lactis homologue of the SPT4 gene of Saccharomyces cerevisiae

被引：2

作者：

Hikkel, I ^{[1
]}

Gbelská, Y ^{[1
]}

Subík, J ^{[1
]}

机构：

[1] Comenius Univ, Fac Nat Sci, Dept Microbiol & Virol, Bratislava, Slovakia

来源：

CURRENT GENETICS | 1998年 / 34卷 / 05期

关键词：

chromatin; suppression; yeast; transcription elongation;

D O I：

10.1007/s002940050409

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Sequence analysis of a DNA fragment containing the KlCOX18 gene originating from chromosome II of the yeast kluyveromyces lactis revealed the presence of an adjacent open reading; frame (ORF) for a protein exhibiting 78.4% identity with the Saccharomyces cerevisiae Spt4p. Based on the identical length (102 aa) and the conservation of the zinc-finger motif found in Spt4p we named this ORF KlSPT4. When expressed in S. cerevisiae the KlSPT4 gene complemented all spt4 mutant phenotypes. It is proposed that KlSpt4p, like its S. cerevisiae counterpart is a protein involved in the establishment or maintenance of the chromatin structure that influences the expression of many yeast genes.

引用

页码：375 / 378

页数：4

共 15 条

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