Crystal structure of a mammalian voltage-dependent Shaker family K+ channel

被引:1754
作者
Long, SB [1 ]
Campbell, EB [1 ]
MacKinnon, R [1 ]
机构
[1] Rockefeller Univ, Howard Hughes Med Inst, Lab Mol Neurobiol & Biophys, New York, NY 10021 USA
关键词
D O I
10.1126/science.1116269
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Voltage-de pendent potassium ion (K+) channels (Kv channels) conduct K+ ions across the cell membrane in response to changes in the membrane voltage, thereby regulating neuronal excitability by modulating the shape and frequency of action potentials. Here we report the crystal structure, at a resolution of 2.9 angstroms, of a mammalian Kv channel, Kv1.2, which is a member of the Shaker K+ channel family. This structure is in complex with an oxido-reductase beta subunit of the kind that can regulate mammalian Kv channels in their native cell environment. The activation gate of the pore is open. Large side portals communicate between the pore and the cytoplasm. Electrostatic properties of the side portals and positions of the T1 domain and beta subunit are consistent with etectrophysiologicat studies of inactivation gating and with the possibility of K+ channel regulation by the beta subunit.
引用
收藏
页码:897 / 903
页数:7
相关论文
共 54 条
[2]   The voltage sensor in voltage-dependent ion channels [J].
Bezanilla, F .
PHYSIOLOGICAL REVIEWS, 2000, 80 (02) :555-592
[3]   Ribbons [J].
Carson, M .
MACROMOLECULAR CRYSTALLOGRAPHY, PT B, 1997, 277 :493-505
[4]   A POTASSIUM CHANNEL BETA-SUBUNIT RELATED TO THE ALDO-KETO REDUCTASE SUPERFAMILY IS ENCODED BY THE DROSOPHILA HYPERKINETIC LOCUS [J].
CHOUINARD, SW ;
WILSON, GF ;
SCHLIMGEN, AK ;
GANETZKY, B .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (15) :6763-6767
[5]  
CONNOR JA, 1971, J PHYSIOL-LONDON, V213, P1
[6]   Tight steric closure at the intracellular activation gate of a voltage-gated K+ channel [J].
del Camino, D ;
Yellen, G .
NEURON, 2001, 32 (04) :649-656
[7]   Blocker protection in the pore of a voltage-gated K+ channel and its structural implications [J].
del Camino, D ;
Holmgren, M ;
Liu, Y ;
Yellen, G .
NATURE, 2000, 403 (6767) :321-325
[8]   The structure of the potassium channel:: Molecular basis of K+ conduction and selectivity [J].
Doyle, DA ;
Cabral, JM ;
Pfuetzner, RA ;
Kuo, AL ;
Gulbis, JM ;
Cohen, SL ;
Chait, BT ;
MacKinnon, R .
SCIENCE, 1998, 280 (5360) :69-77
[9]   Structure of the cytoplasmic β subunit -: T1 assembly of voltage-dependent K+ channels [J].
Gulbis, JM ;
Zhou, M ;
Mann, S ;
MacKinnon, R .
SCIENCE, 2000, 289 (5476) :123-127
[10]   Structure of a voltage-dependent K+ channel β subunit [J].
Gulbis, JM ;
Mann, S ;
MacKinnon, R .
CELL, 1999, 97 (07) :943-952