Cold shock and cold acclimation proteins in the psychrotrophic bacterium Arthrobacter globiformis SI55

被引:108
作者
Berger, F
Morellet, N
Menu, F
Potier, P
机构
[1] UNIV LYON 1, LAB ECOL MICROBIENNE SOL, UMR CNRS 5557, F-69622 VILLEURBANNE, FRANCE
[2] UNIV LYON 1, LAB BIOMET GENET & BIOL POPULAT, UMR CNRS 5558, F-69622 VILLEURBANNE, FRANCE
关键词
D O I
10.1128/jb.178.11.2999-3007.1996
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The psychrotrophic bacterium Arthrobacter globiformis SI55 was grown at 4 and 25 degrees C, and the cell protein contents were analyzed by two-dimensional electrophoresis. Cells subjected to cold shocks of increasing magnitude were also analyzed. Correspondence analysis of protein appearance distinguished four groups of physiological significance. Group I contained cold shock proteins (Csps) overexpressed only after a large temperature downshift. Group II contained Csps with optimal expression after mild shocks. Group III contained proteins overexpressed after all cold shocks. These last proteins were also overexpressed in cells growing at 4 degrees C and were considered to be early cold acclimation proteins (Caps). Group IV contained proteins which were present at high concentrations only in 4 degrees C steady-state cells and appeared to be late Caps. A portion of a gene very similar to the Escherichia coli cspA gene (encoding protein CS7.4) was identified. A synthetic peptide was used to produce an antibody which detected a CS7.4-like protein (A9) by immunoblotting two-dimensional electrophoresis gels of A. globiformis SI55 total proteins. Unlike mesophilic microorganisms, this CS7.4-like protein was still produced during prolonged growth at low temperature, and it might have a particular adaptive function needed for balanced growth under harsh conditions. However, A9 was induced at high temperature by chloramphenicol, suggesting that CS7.4-like proteins have a more general role than their sole implication in cold acclimation processes.
引用
收藏
页码:2999 / 3007
页数:9
相关论文
共 48 条
[11]   A CONTINOUS CULTURE STUDY OF AN OBLIGATELY PSYCHROPHILIC PSEUDOMONAS SPECIES [J].
HARDER, W ;
VELDKAMP, H .
ARCHIV FUR MIKROBIOLOGIE, 1967, 59 (1-3) :123-+
[12]   EFFECT OF GROWTH TEMPERATURES ON THE PROTEIN-LEVELS IN A PSYCHROTROPHIC BACTERIUM, PSEUDOMONAS-FRAGI [J].
HEBRAUD, M ;
DUBOIS, E ;
POTIER, P ;
LABADIE, J .
JOURNAL OF BACTERIOLOGY, 1994, 176 (13) :4017-4024
[13]   MOLECULAR CHAPERONE FUNCTIONS OF HEAT-SHOCK PROTEINS [J].
HENDRICK, JP ;
HARTL, FU .
ANNUAL REVIEW OF BIOCHEMISTRY, 1993, 62 :349-384
[14]   LEVELS OF MAJOR PROTEINS OF ESCHERICHIA-COLI DURING GROWTH AT DIFFERENT TEMPERATURES [J].
HERENDEEN, SL ;
VANBOGELEN, RA ;
NEIDHARDT, FC .
JOURNAL OF BACTERIOLOGY, 1979, 139 (01) :185-194
[15]  
HIGGINS CF, 1990, BACTERIAL CHROMOSOME, P421
[16]   A PHYSIOLOGICAL-ROLE FOR DNA SUPERCOILING IN THE OSMOTIC REGULATION OF GENE-EXPRESSION IN S-TYPHIMURIUM AND ESCHERICHIA-COLI [J].
HIGGINS, CF ;
DORMAN, CJ ;
STIRLING, DA ;
WADDELL, L ;
BOOTH, IR ;
MAY, G ;
BREMER, E .
CELL, 1988, 52 (04) :569-584
[17]  
Hill M. O., 1974, Applied Statistics, V23, P340, DOI 10.2307/2347127
[18]   RECIPROCAL AVERAGING - EIGENVECTOR METHOD OF ORDINATION [J].
HILL, MO .
JOURNAL OF ECOLOGY, 1973, 61 (01) :237-244
[19]  
INGRAHAM J. L., 1963, RECENT PROGR MICROBIOL, V8, P201
[20]   CHLORAMPHENICOL INDUCES THE TRANSCRIPTION OF THE MAJOR COLD SHOCK GENE OF ESCHERICHIA-COLI, CSPA [J].
JIANG, WN ;
JONES, P ;
INOUYE, M .
JOURNAL OF BACTERIOLOGY, 1993, 175 (18) :5824-5828