Appearance of intratypic recombination of enterovirus 71 in Taiwan from 2002 to 2005

被引：62

作者：

Huang, Shih-Chen ^{[2
]}

Hsu, Yun-Wei ^{[3
]}

Wang, Hsuan-Chen ^{[3
]}

Huang, Sheng-Wen ^{[4
]}

Kiang, David

Tsai, Huey-Pin ^{[5
]}

Wang, Shih-Ming ^{[6
]}

Liu, Ching-Chuan ^{[6
]}

Lin, Kui-Hsiang ^{[7
]}

Su, Ih-Jen ^{[3
]}

Wang, Jen-Ren ^{[1
,2
,3
,5
]}

机构：

[1] Natl Cheng Kung Univ, Coll Med, Dept Med Lab Sci & Biotechnol, Tainan 701, Taiwan

[2] Natl Cheng Kung Univ, Coll Med, Inst Mol Med, Tainan 701, Taiwan

[3] Natl Hlth Res Inst, Div Clin Res, Tainan, Taiwan

[4] Natl Cheng Kung Univ, Coll Med, Inst Basic Med Sci, Tainan, Taiwan

[5] Natl Cheng Kung Univ, Dept Pathol, Tainan 70101, Taiwan

[6] Natl Cheng Kung Univ, Coll Med, Dept Pediat, Tainan 70101, Taiwan

[7] Kaohsiung Med Univ, Dept Clin Lab, Kaohsiung, Taiwan

来源：

VIRUS RESEARCH | 2008年 / 131卷 / 02期

关键词：

enterovirus; 71; phylogenetic analysis; recombination;

D O I：

10.1016/j.virusres.2007.10.002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Genetic recombination is a well-known phenomenon for enteroviruses. In this study, we determined the phylogenetic relationships of five distinct regions of the EV71 genome for 73 EV71 isolates from 1986 and from 1998 to 2005 in Taiwan. Phylogenetic analyses showed that the 5'-UTR, VP4-VP2, VP1, and 3D regions of EV71 isolated in 2004 and 2005 were grouped into genotype C. However, the 2B region of these isolates differed in that it grouped with genotype B, indicating recombination within EV71 had occurred. This intratypic recombination was first seen in 2002 and became predominant in 2004 and 2005. The simplot and bootscan analyses identified two recombination points located at the 3'-termini of the 2A and 3C regions. This intratypic recombination was identified among naturally circulating EV71 isolates in Taiwan, therefore, it suggests that nonstructural genes may recombine to produce new EV71 variants. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：250 / 259

页数：10

共 46 条

[1] PICORNAVIRAL 3C CYSTEINE PROTEINASES HAVE A FOLD SIMILAR TO CHYMOTRYPSIN-LIKE SERINE PROTEINASES [J].

ALLAIRE, M ;

CHERNAIA, MM ;

MALCOLM, BA ;

JAMES, MNG .

NATURE, 1994, 369 (6475) :72-76

[2] Molecular analysis of the echovirus 18 prototype - Evidence of interserotypic recombination with echovirus 9 [J].

Andersson, P ;

Edman, K ;

Lindberg, AM .

VIRUS RESEARCH, 2002, 85 (01) :71-83

[3] Regulation of picornavirus gene expression [J].

Bedard, KM ;

Semler, BL .

MICROBES AND INFECTION, 2004, 6 (07) :702-713

[4] Molecular epidemiology and evolution of enterovirus 71 strains isolated from 1970 to 1998 [J].

Brown, BA ;

Oberste, MS ;

Alexander, JP ;

Kennett, ML ;

Pallansch, MA .

JOURNAL OF VIROLOGY, 1999, 73 (12) :9969-9975

[5] Individual expression of poliovirus 2Apro and 3Cpro induces activation of caspase-3 and PARP cleavage in HeLa cells [J].

Calandria, C ;

Irurzun, A ;

Barco, A ;

Carrasco, L .

VIRUS RESEARCH, 2004, 104 (01) :39-49

[6]

CAMMACK N, 1988, VIROLOGY, V167, P507, DOI 10.1016/0042-6822(88)90113-4

[7]

Chan YF, 2004, EMERG INFECT DIS, V10, P1468

[8] Human enterovirus 71 subgenotype B3 lacks coxsackievirus A16-like neurovirulence in mice infection [J].

Chan, Yoke-Fun ;

AbuBakar, Sazaly .

VIROLOGY JOURNAL, 2005, 2 (1)

[9] MEMBRANE REARRANGEMENT AND VESICLE INDUCTION BY RECOMBINANT POLIOVIRUS 2C AND 2BC IN HUMAN-CELLS [J].

CHO, MW ;

TETERINA, N ;

EGGER, D ;

BIENZ, K ;

EHRENFELD, E .

VIROLOGY, 1994, 202 (01) :129-145

[10] Genomic features of intertypic recombinant Sabin poliovirus strains excreted by primary vaccinees [J].

Cuervo, NS ;

Guillot, S ;

Romanenkova, N ;

Combiescu, M ;

Aubert-Combiescu, A ;

Seghier, M ;

Caro, V ;

Crainic, R ;

Delpeyroux, F .

JOURNAL OF VIROLOGY, 2001, 75 (13) :5740-5751

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