Investigations into the interaction between tumor-inhibiting ruthenium(III) complexes and nucleotides by capillary electrophoresis

被引：34

作者：

Küng, A ^{[1
]}

Pieper, T ^{[1
]}

Keppler, BK ^{[1
]}

机构：

[1] Univ Vienna, Inst Inorgan Chem, A-1090 Vienna, Austria

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2001年 / 759卷 / 01期

关键词：

cancer; ruthenium(III); nucleotides;

D O I：

10.1016/S0378-4347(01)00205-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Ruthenium(III) complexes of the general formula HL[RuCl4L2], with two trans-standing heterocyclic ligands L bound to ruthenium via nitrogen, show remarkable activity in different tumor models. To obtain a deeper insight into the mode of action of this class of anticancer compounds, we investigated the interaction of HIm tmns-[RuCl4(im)(2)] (im, imidazole) and HInd trans-[RuCl4(ind)(2)] (ind, indazole) with all four nucleoside monophosphates in buffered solution by means of capillary electrophoresis. A preference for GMP- and AMP-coordination was found. A decrease of the pH resulted in a significantly increased amount of bound nucleotide. This feature seems to be interesting with regard to the lower pH values in solid tumors. (C) 2001 Elsevier Science B.V. All rights reserved.

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页码：81 / 89

页数：9

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