Identification of bovine sperm head for morphometry analysis in quantitative phase-contrast holographic microscopy

被引：61

作者：

Memmolo, P. ^{[1
,3
]}

Di Caprio, G. ^{[4
]}

Distante, C. ^{[2
]}

Paturzo, M. ^{[1
]}

Puglisi, R. ^{[5
]}

Balduzzi, D. ^{[5
]}

Galli, A. ^{[5
]}

Coppola, G. ^{[4
]}

Ferraro, P. ^{[1
]}

机构：

[1] Ist Nazl Ottica, CNR, I-80078 Pozzuoli, NA, Italy

[2] Ist Nazl Ottica, CNR, I-73010 Arnesano, LE, Italy

[3] Univ Naples Federico II, DIBET, I-80125 Naples, Italy

[4] Ist Microelettron & Microsistemi, CNR, I-80131 Naples, Italy

[5] Ist Sperimentale Italiano Lazzaro Spallanzani, I-26027 Rivolta Dadda, CR, Italy

来源：

OPTICS EXPRESS | 2011年 / 19卷 / 23期

关键词：

IN-LINE HOLOGRAPHY; RECONSTRUCTION; VISUALIZATION; SPERMATOZOA; FERTILITY; EFFICIENT; BINDING; SPACE;

D O I：

10.1364/OE.19.023215

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

An investigation is reported of the identification and measurement of region of interest (ROI) in quantitative phase-contrast maps of biological cells by digital holographic microscopy. In particular, two different methods have been developed for in vitro bull sperm head morphometry analysis. We show that semen analysis can be accomplished by means of the proposed techniques. Extraction and measurement of various parameters are performed. It is demonstrated that both proposed methods are efficient to skim the data set in a preselective analysis for discarding anomalous data. (C) 2011 Optical Society of America

引用

页码：23215 / 23226

页数：12

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