Membrane ion-exchange chromatography for process-scale antibody purification

被引：175

作者：

Knudsen, HL ^{[1
]}

Fahrner, RL ^{[1
]}

Xu, Y ^{[1
]}

Norling, LA ^{[1
]}

Blank, GS ^{[1
]}

机构：

[1] Genentech Inc, Dept Recovery Sci, S San Francisco, CA 94080 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2001年 / 907卷 / 1-2期

关键词：

preparative chromatography; membranes; breakthrough volumes; antibodies;

D O I：

10.1016/S0021-9673(00)01041-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The large-scale production of recombinant monoclonal antibodies demands economical purification processes with high throughputs. The potential for ion-exchange membrane adsorbers to replace traditional ion-exchange columns was evaluated. Breakthrough capacities of commercially available cation-exchange membranes were determined as a function of Row-rate and layer number. Due to economic and process restrictions, cation-exchange membranes may not currently be advantageous for process-scale antibody purification in a bind and elute mode. However, anion-exchange membranes in a how-through mode may provide a reasonable alternative to columns for the removal of low levels of impurities such as DNA, host cell protein, and virus. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：145 / 154

页数：10

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