Dicyanostilbene-Derived Two-Photon Fluorescence Probe for Free Zinc Ions in Live Cells and Tissues with a Large Two-Photon Action Cross Section

被引：41

作者：

Huang, Chibao ^{[1
]}

Qu, Junle

Qi, Jing

Yan, Meng

Xu, Gaixia

机构：

[1] Xiangtan Univ, Coll Chem, Minist Educ, Key Lab Environm Friendly Chem & Applicat, Xiangtan 411105, Peoples R China

来源：

ORGANIC LETTERS | 2011年 / 13卷 / 06期

基金：

中国博士后科学基金;

关键词：

TWIN-CYANO-STILBENE; ABSORPTION; ZN2+; COORDINATION; SENSOR;

D O I：

10.1021/ol200146j

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A novel two-photon fluorescence probe for Zn(2+) derived from dicyanostilbene as a two-photon fluorophore and 4-(pyridine-2-ylmethyl)piperazine as a novel Zn(2+) ligand was developed. The probe shows a 72.5-fold fluorescence enhancement In response to Zn(2+), a large two-photon action cross-section (580 GM), a noncytotoxic effect, and pH insensitivity in the biologically relevant range, and its dissociation constant (K(d)(TP)) is 0.52 +/- 0.01 mu M. The probe can selectively detect free Zn(2+) ions in live cells for 1500 s or so and in living tissues at a depth of 80-150 mu m without Interference from other metal Ions and the membrane-bound probes.

引用

页码：1462 / 1465

页数：4

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