Identification and characterization of a homologue of the pro-inflammatory cytokine Macrophage Migration Inhibitory Factor in the tick, Amblyomma americanum

被引：71

作者：

Jaworski, DC

Jasinskas, A

Metz, CN

Bucala, R

Barbour, AG

机构：

[1] Univ Calif Irvine, Dept Microbiol & Mol Genet, Irvine, CA 92697 USA

[2] Univ Calif Irvine, Dept Med, Irvine, CA 92717 USA

[3] Picower Inst Med Res, Manhasset, NY USA

来源：

INSECT MOLECULAR BIOLOGY | 2001年 / 10卷 / 04期

关键词：

Amblyomma tick; macrophage inhibiting factor; cytokine; salivary gland; vector;

D O I：

10.1046/j.0962-1075.2001.00271.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Studying tick feeding and digestion, we discovered in a cDNA library from partially fed Amblyomma americanum ticks the first known arthropod homologue of a human cytokine, the pro-inflammatory Macrophage Migration Inhibitory Factor (MIF). The tick origin of the MIF cDNA clone was confirmed by sequencing a genomic fragment that contained the full-length tick MIF gene with two introns. Antiserum to a tick MIF-specific peptide as well as antiserum to complete tick MIF revealed the expression of tick MIF in the salivary gland and midgut tissues of A. americanum, ticks. In an in vitro functional assay, recombinant tick MIF inhibited the migration of human macrophages to the same extent that recombinant human MIF did.

引用

页码：323 / 331

页数：9

共 34 条

[1] An essential regulatory role for macrophage migration inhibitory factor in T-cell activation [J].