A phosphate transporter gene from the extra-radical mycelium of an arbuscular mycorrhizal fungus Glomus intraradices is regulated in response to phosphate in the environment

被引:181
作者
Maldonado-Mendoza, IE [1 ]
Dewbre, GR [1 ]
Harrison, MJ [1 ]
机构
[1] Samuel Roberts Noble Fdn Inc, Ardmore, OK 73401 USA
关键词
D O I
10.1094/MPMI.2001.14.10.1140
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The majority of vascular flowering plants are able to form symbiotic associations with arbuscular mycorrhizal fungi. These symbioses, termed arbuscular mycorrhizas, are mutually beneficial, and the fungus delivers phosphate to the plant while receiving carbon. In these symbioses, phosphate uptake by the arbuscular mycorrhizal fungus is the first step in the process of phosphate transport to the plant. Previously, we cloned a phosphate transporter gene involved in this process. Here, we analyze the expression and regulation of a phosphate transporter gene (GiPT) in the extra-radical mycelium of the arbuscular mycorrhizal fungus Glomus intraradices during mycorrhizal association with carrot or Medicago truncatula roots. These analyses reveal that GiPT expression is regulated in response to phosphate concentrations in the environment surrounding the extra-radical hyphae and modulated by the overall phosphate status of the mycorrhiza. Phosphate concentrations, typical of those found in the soil solution, result in expression of GiPT These data imply that G. intraradices can perceive phosphate levels in the external environment but also suggest the presence of an internal phosphate sensing mechanism.
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页码:1140 / 1148
页数:9
相关论文
共 47 条
[41]   Enhanced hyphal growth and spore production of the arbuscular mycorrhizal fungus Glomus intraradices in an in vitro system in the absence of host roots [J].
StArnaud, M ;
Hamel, C ;
Vimard, B ;
Caron, M ;
Fortin, JA .
MYCOLOGICAL RESEARCH, 1996, 100 :328-332
[42]   REGULATION OF INORGANIC-PHOSPHATE TRANSPORT-SYSTEMS IN SACCHAROMYCES CEREVISIAE [J].
TAMAI, Y ;
TOHE, A ;
OSHIMA, Y .
JOURNAL OF BACTERIOLOGY, 1985, 164 (02) :964-968
[43]   KINETICS OF PHOSPHORUS UPTAKE BY THE GERM-TUBES OF THE VESICULAR ARBUSCULAR MYCORRHIZAL FUNGUS, GIGASPORA-MARGARITA [J].
THOMSON, BD ;
CLARKSON, DT ;
BRAIN, P .
NEW PHYTOLOGIST, 1990, 116 (04) :647-653
[44]   Transformation of Medicago truncatula via infiltration of seedlings or flowering plants with Agrobacterium [J].
Trieu, AT ;
Burleigh, SH ;
Kardailsky, IV ;
Maldonado-Mendoza, IE ;
Versaw, WK ;
Blaylock, LA ;
Shin, HS ;
Chiou, TJ ;
Katagi, H ;
Dewbre, GR ;
Weigel, D ;
Harrison, MJ .
PLANT JOURNAL, 2000, 22 (06) :531-541
[45]   Novel genes induced during an arbuscular mycorrhizal (AM) symbiosis formed between Medicago truncatula and Glomus versiforme [J].
van Buuren, ML ;
Maldonado-Mendoza, IE ;
Trieu, AT ;
Blaylock, LA ;
Harrison, MJ .
MOLECULAR PLANT-MICROBE INTERACTIONS, 1999, 12 (03) :171-181
[46]   Mycorrhizal fungal diversity determines plant biodiversity, ecosystem variability and productivity [J].
van der Heijden, MGA ;
Klironomos, JN ;
Ursic, M ;
Moutoglis, P ;
Streitwolf-Engel, R ;
Boller, T ;
Wiemken, A ;
Sanders, IR .
NATURE, 1998, 396 (6706) :69-72
[47]   A PHOSPHATE-REPRESSIBLE, HIGH-AFFINITY PHOSPHATE PERMEASE IS ENCODED BY THE PHO-5(+) GENE OF NEUROSPORA-CRASSA [J].
VERSAW, WK .
GENE, 1995, 153 (01) :135-139