Cryo-Electron Tomography: Can it Reveal the Molecular Sociology of Cells in Atomic Detail?

被引：217

作者：

Beck, Martin ^{[1
]}

Baumeister, Wolfgang ^{[2
]}

机构：

[1] European Mol Biol Lab, Struct & Computat Biol Unit, D-69117 Heidelberg, Germany

[2] Max Planck Inst Biochem, Dept Mol Struct Biol, D-82152 Martinsried, Planegg, Germany

来源：

TRENDS IN CELL BIOLOGY | 2016年 / 26卷 / 11期

关键词：

NUCLEAR-PORE COMPLEX; PATHOGEN LEPTOSPIRA-INTERROGANS; 26S PROTEASOME; STRUCTURAL-ANALYSIS; CRYO-EM; ELECTRON TOMOGRAPHY; ANGSTROM RESOLUTION; VISUAL PROTEOMICS; MASS-SPECTROMETRY; COATED VESICLES;

D O I：

10.1016/j.tcb.2016.08.006

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

Traditionally, macromolecular structure determination is performed ex situ, that is, with purified materials. But, there are strong incentives to develop approaches to study them in situ in their native functional context. In recent years, cryo-electron tomography (cryo-ET) has emerged as a powerful method for visualizing the molecular organization of unperturbed cellular landscapes with the potential to attain near-atomic resolution. Here, we review recent work on several macromolecular assemblies, demonstrating the power of in situ studies. We also highlight technical challenges and discuss ways to meet them.

引用

页码：825 / 837

页数：13

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