The quantitative proteome of a human cell line

被引：627

作者：

Beck, Martin ^{[2
]}

Schmidt, Alexander ^{[3
]}

Malmstroem, Johan ^{[4
,5
]}

Claassen, Manfred ^{[6
]}

Ori, Alessandro ^{[2
]}

Szymborska, Anna ^{[2
]}

Herzog, Franz ^{[1
]}

Rinner, Oliver ^{[5
]}

Ellenberg, Jan ^{[2
]}

Aebersold, Ruedi ^{[1
,7
,8
]}

机构：

[1] ETH, Dept Biol, Inst Mol Syst Biol, CH-8093 Zurich, Switzerland

[2] European Mol Biol Lab, Heidelberg, Germany

[3] Univ Basel, Biozentrum, Basel, Switzerland

[4] BMC, Dept Immunotechnol, Lund, Sweden

[5] Biognosys AG, Schlieren, Switzerland

[6] ETH, Dept Comp Sci, CH-8093 Zurich, Switzerland

[7] Competence Ctr Syst Physiol & Metab Dis, Zurich, Switzerland

[8] Univ Zurich, Dept Sci, Zurich, Switzerland

来源：

MOLECULAR SYSTEMS BIOLOGY | 2011年 / 7卷

基金：

欧洲研究理事会;

关键词：

mass spectrometry; protein abundance; proteomics; ABSOLUTE QUANTIFICATION; COVERAGE PREDICTION; MESSENGER-RNA; PROTEINS; GENOME; EXPRESSION; IDENTIFICATIONS; FRACTIONATION; SEQUENCES; PEPTIDES;

D O I：

10.1038/msb.2011.82

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

The generation of mathematical models of biological processes, the simulation of these processes under different conditions, and the comparison and integration of multiple data sets are explicit goals of systems biology that require the knowledge of the absolute quantity of the system's components. To date, systematic estimates of cellular protein concentrations have been exceptionally scarce. Here, we provide a quantitative description of the proteome of a commonly used human cell line in two functional states, interphase and mitosis. We show that these human cultured cells express at least similar to 10 000 proteins and that the quantified proteins span a concentration range of seven orders of magnitude up to 20 000 000 copies per cell. We discuss how protein abundance is linked to function and evolution. Molecular Systems Biology 7: 549; published online 8 November 2011; doi:10.1038/msb.2011.82

引用

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