Universal labeling chemistry for nucleic acid detection on DNA-Arrays

被引：6

作者：

Bernal-Méndez, E ^{[1
]}

Tora, C

Sothier, I

Kotera, M

Troesch, A

Laayoun, A

机构：

[1] BioMerieux SA, Chem Orne, F-69280 Marcy Letoile, France

[2] Univ Grenoble 1, CNRS, LEDSS, UMR 5616, Grenoble, France

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2003年 / 22卷 / 5-8期

关键词：

labeling; nucleic acids; diazomethyl; DNA-chip;

D O I：

10.1081/NCN-120023090

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We show here a new and efficient aqueous chemistry for labeling of any class of nucleic acids for their detection on DNA chip. The labels contain a diazo function as reactive moiety and biotin as detectable unit. The highly selective reaction of diazo group on the phosphate does not disrupt base pairing recognition and hybridization specificity.

引用

页码：1647 / 1649

页数：3

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