Integrin-mediated invasion of Staphylococcus aureus into human cells requires Src family protein-tyrosine kinases

被引:98
作者
Agerer, F
Michel, A
Ohlsen, K
Hauck, CR
机构
[1] Univ Wurzburg, Zentrum Infekt Forsch, D-97070 Wurzburg, Germany
[2] Univ Wurzburg, Inst Mol Infekt Biol, D-97070 Wurzburg, Germany
关键词
D O I
10.1074/jbc.M302096200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Staphylococcus aureus, a common cause of nosocomial infections, is able to invade eukaryotic cells by indirectly engaging beta(1) integrin-containing host receptors, whereas non-pathogenic Staphylococcus carnosus is not invasive. Here, we identify intracellular signals involved in integrin-initiated internalization of S. aureus. In particular, the host cell actin cytoskeleton and Src family protein-tyrosine kinases (PTKs) are essential to mediate S. aureus invasion. Src PTKs are activated in response to pathogenic S. aureus, but not S. carnosus. In addition, pharmacological and genetic interference with Src PTK function reduces bacterial internalization. Importantly, Src PTK-deficient cells are resistant to S. aureus invasion, demonstrating the essentiality of host Src PTKs in integrin-mediated uptake of this pathogen.
引用
收藏
页码:42524 / 42531
页数:8
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