Cloning and characterization of ZmPIP1-5b, an aquaporin transporting water and urea

被引：92

作者：

Gaspar, M ^{[1
]}

Bousser, A ^{[1
]}

Sissoëff, I ^{[1
]}

Roche, O ^{[1
]}

Hoarau, J ^{[1
]}

Mahé, A ^{[1
]}

机构：

[1] Univ Paris 11, CNRS, UMR 8618, Inst Biotechnol Plantes,Lab Struct & Metab Plante, F-91405 Orsay, France

来源：

PLANT SCIENCE | 2003年 / 165卷 / 01期

关键词：

Zea mays L; PIP; roots;

D O I：

10.1016/S0168-9452(03)00117-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA, ZmPIP1-5b, isolated from a nitrate-treated maize roots cDNA library has 95.2% sequence identity to ZmPIP1-5. However, sequence divergences are not uniformly distributed, but are mainly observed in 3' and 5' non-coding regions (82.7 and 77.3%, respectively). The coding regions differ by two nucleotides over 864, but ZmPIP1-5b and ZmPIP1-5 encode identical proteins. The deduced ZmPIP1-5 protein is 288 amino acids in length, contains six putative transmembrane domains and the MIP signature NPA. ZmPIP1-5, like other proteins belonging to PIPI subfamily, has a low aquaporin activity when expressed in Xenopus oocytes. However, a special feature of ZmPIP1-5, when compared with other plant PIPs, is its capacity to transport urea. ZmPIP1-5 is preferentially expressed in roots and addition of KNO3 to previously nitrogen-starved maize plants induced the transcript level of this gene. Further-more, ZmPIP1-5 expression oscillated during the day-night cycle. In situ hybridization showed that this regulation is tissue specific. Modulation of ZmPIP1-5 expression by KNO3 and during the day-night cycle is discussed in regards to root water permeability changes, (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：21 / 31

页数：11

共 37 条

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