Separation of diastereomers of flavanone-7-O-glycosides by capillary electrophoresis using sulfobutyl ether-β-cyclodextrin as the selector

被引:24
作者
Aturki, Z [1 ]
Sinibaldi, M [1 ]
机构
[1] CNR, Area Ric Roma 1, Ist Metodol Chim, I-00016 Rome, Italy
来源
JOURNAL OF SEPARATION SCIENCE | 2003年 / 26卷 / 9-10期
关键词
diastereomer separation; capillary electrophoresis; sulfobutyl ether-beta-cyclodextrin; flavanone-7-O-glycosides; citrus juices; chiral flavanones;
D O I
10.1002/jssc.200301480
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A method was developed for the separation of diastereomers of flavanone-7-O-glycosides by capillary electrophoresis using sulfobutyl ether-beta-cyclodextrin (SBE-beta-CD) in the background electrolyte. The effect of the concentration of the CID additive, buffer pH, and organic modifier on the migration times and resolution for five flavanone glycosides (naringin, hesperidin, neohesperidin, narirutin, and eriocitrin) was studied. Baseline separations of these compounds as pairs of diastereoisomers were achieved with 20 mM tetraborate buffer at pH 7 containing 5 mg/mL of SBE-beta-CD and 10% (v/v) of methanol. The developed method was used for the qualitative analysis of the diastereomeric composition of the major flavanone glycosides in different citrus juices. The ability of SBE-beta-CD to discriminate flavanone enantiomers was also investigated.
引用
收藏
页码:844 / 850
页数:7
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