Separation of diastereomers of flavanone-7-O-glycosides by capillary electrophoresis using sulfobutyl ether-β-cyclodextrin as the selector

A method was developed for the separation of diastereomers of flavanone-7-O-glycosides by capillary electrophoresis using sulfobutyl ether-beta-cyclodextrin (SBE-beta-CD) in the background electrolyte. The effect of the concentration of the CID additive, buffer pH, and organic modifier on the migration times and resolution for five flavanone glycosides (naringin, hesperidin, neohesperidin, narirutin, and eriocitrin) was studied. Baseline separations of these compounds as pairs of diastereoisomers were achieved with 20 mM tetraborate buffer at pH 7 containing 5 mg/mL of SBE-beta-CD and 10% (v/v) of methanol. The developed method was used for the qualitative analysis of the diastereomeric composition of the major flavanone glycosides in different citrus juices. The ability of SBE-beta-CD to discriminate flavanone enantiomers was also investigated.