In vivo monitoring of the thiols in rat striatum by liquid chromatography with amperometric detection at a functionalized multi-wall carbon nanotubes modified electrode

A new chemically modified electrode (CME) was fabricated, which was based on the immobilization of multi-wall carbon nanotubes fuctionalized with carboxylic group (MWNT-COOH). The results indicated that the CME exhibited efficiently electrocatalytic oxidation for L-cysteine and glutathione with relatively high sensitivity, stability and long-life. Coupled with HPLC, the MWNT-COOH CME was utilized for amperometric detection of the thiols. The peak currents of L-cysteine and glutathione were linear to their concentrations ranging from 3.0x10(-7) to 1.0x10(-3) mol/L with the calculated detection limit (S/N=3) of 1.2x10(-7), 2.2x10(-7) mol/L, respectively. The method had been successfully applied to assess the contents of L-cysteine and glutathione in rat striatal microdialysates.