Surface structure and nanomechanical properties of Shewanella putrefaciens bacteria at two pH values (4 and 10) determined by atomic force microscopy

被引：101

作者：

Gaboriaud, F ^{[1
]}

Bailet, S ^{[1
]}

Dague, E ^{[1
]}

Jorand, F ^{[1
]}

机构：

[1] Univ Nancy 1, CNRS, UMR 7564, Lab Chim Phys & Microbiol Environm, F-54600 Villers Les Nancy, France

来源：

JOURNAL OF BACTERIOLOGY | 2005年 / 187卷 / 11期

关键词：

D O I：

10.1128/JB.187.11.3864-3868.2005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The nanomechanical properties of gram-negative bacteria (Shewanelia putrefaciens) were investigated in situ in aqueous solutions at two pH values, specifically, 4 and 10, by atomic force microscopy (AFM). For both pH values, the approach force curves exhibited subsequent nonlinear and linear regimens that were related to the progressive indentation of the AFM tip in the bacterial cell wall, including a priori polymeric fringe (nonlinear part), while the linear part was ascribed to compression of the plasma membrane. These results indicate the dynamic of surface ultrastructure in response to changes in pH, leading to variations in nanomechanical properties, such as the Young's modulus and the bacterial spring constant.

引用

页码：3864 / 3868

页数：5

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