Simple and sensitive electrode design for microchip electrophoresis/electrochemistry

被引:79
作者
Liu, Y [1 ]
Vickers, JA [1 ]
Henry, CS [1 ]
机构
[1] Colorado State Univ, Dept Chem, Ft Collins, CO 80523 USA
关键词
D O I
10.1021/ac0350357
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A simple and sensitive electrode design for microchip capillary electrophoresis/electrochemistty (CE-EC) is presented. The system employs metal microwires as the working electrodes for electrochemical detection. Two general approaches for integration of electrodes in microchip CE-EC are commonly used, end-channel and microfabrication. The end-channel approach allows electrode cleaning and the use of chemically modified electrodes; however, the designs generally lack portability and the ability to incorporate multiple electrodes. Microfabrication allows the incorporation of multiple electrodes on-chip and is easily made portable; however, it requires the use of expensive metallization and clean room facilities, and integration of more than one electrode material is challenging. The reported approach aligns a solid metal microwire through the separation channel allowing integration of multiple electrodes and the use of different electrode materials without sacrificing the portability. A detection limit of 100 nM for dopamine was achieved without the use of a decoupler as a result of a higher collection efficiency with the new design.
引用
收藏
页码:1513 / 1517
页数:5
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