A Single Point Mutation Creating a Furin Cleavage Site in the Spike Protein Renders Porcine Epidemic Diarrhea Coronavirus Trypsin Independent for Cell Entry and Fusion

被引：41

作者：

Li, Wentao ^{[1
,2
]}

Wicht, Oliver ^{[1
]}

van Kuppeveld, Frank J. M. ^{[1
]}

He, Qigai ^{[2
]}

Rottier, Peter J. M. ^{[1
]}

Bosch, Berend-Jan ^{[1
]}

机构：

[1] Univ Utrecht, Fac Vet Med, Dept Infect Dis & Immunol, Div Virol, Utrecht, Netherlands

[2] Huazhong Agr Univ, Coll Vet Med, State Key Lab Agr Microbiol, Wuhan, Peoples R China

来源：

JOURNAL OF VIROLOGY | 2015年 / 89卷 / 15期

关键词：

RESPIRATORY SYNDROME CORONAVIRUS; INFECTIOUS-BRONCHITIS VIRUS; PROTEOLYTIC CLEAVAGE; MURINE CORONAVIRUS; ACTIVATION; E2-GLYCOPROTEIN; CULTURE; DOMAIN;

D O I：

10.1128/JVI.00356-15

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The emerging porcine epidemic diarrhea virus (PEDV) requires trypsin supplementation to activate its S protein for membrane fusion and virus propagation in cell culture. By substitution of a single amino acid in the S protein, we created a recombinant PEDV with an artificial furin protease cleavage site N terminal of the putative fusion peptide (PEDV-SFCS). PEDV-SFCS exhibited trypsin-independent cell-cell fusion and was able to replicate in culture cells independently of trypsin, though to low titer.

引用

页码：8077 / 8081

页数：5

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