Effects of chronic AICAR treatment on fiber composition, enzyme activity, UCP3, and PGC-1 in rat muscles

被引：214

作者：

Suwa, M

Nakano, H

Kumagai, S ^{[1
]}

机构：

[1] Kyushu Univ, Inst Hlth Sci, Fukuoka 8168580, Japan

[2] Nakamura Gakuen Univ, Dept Human Dev, Jonan Ku, Fukuoka 8140198, Japan

来源：

JOURNAL OF APPLIED PHYSIOLOGY | 2003年 / 95卷 / 03期

关键词：

5 '-AMP-activated protein kinase; antioxidant capacity; mitochondrial enzymes; muscle fiber type composition; peroxisome proliferator-activated receptor-gamma coactivator-1 alpha; 5-aminoimidazole-4-carboxamide-1-beta-D; ribofuranoside; uncoupling protein 3;

D O I：

10.1152/japplphysiol.00349.2003

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

This study was designed to determine the histological and metabolic effects of the administration of 5'-AMP-activated protein kinase (AMPK) activator 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (AICAR) for 14 successive days. AICAR treatment caused a significant decrease in the percentage of type IIB fibers and the concomitant increase in the percentage of type IIX fibers in extensor digitorum longus (EDL) muscle. The capillary density and the capillary-to-fiber ratio were not altered by AICAR. AICAR treatment increased the glycolytic and oxidative enzyme activities but not the antioxidant enzyme activities. The AICAR treatment increased the uncoupling protein 3 (UCP3) level in EDL and the peroxisome proliferator-activated receptor-gamma coactivator-1alpha protein level in the soleus and EDL muscles, whereas the myogenin level was not altered by AICAR. These results seem to imply that the chronic activation of AMPK alters such muscle histochemical and metabolic characteristics.

引用

页码：960 / 968

页数：9

共 58 条

[31]

Merrill GF, 1997, AM J PHYSL ENDOCRINO, P273

[32] Restoration of insulin-sensitive glucose transporter (GLUT4) gene expression in muscle cells by the transcriptional coactivator PGC-1 [J].