Methods to improve the yield and quality of DNA from dried and processed figs

被引：17

作者：

Di Bernardo, G

Galderisi, U

Cipollaro, M

Cascino, A

机构：

[1] Univ Naples 2, Dept Expt Med, Sect Biotechnol & Mol Biol, I-80138 Naples, Italy

[2] Univ Naples 2, CRISCEB, I-80138 Naples, Italy

来源：

BIOTECHNOLOGY PROGRESS | 2005年 / 21卷 / 02期

关键词：

D O I：

10.1021/bp049710p

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We describe here a molecular method that can be used to detect genome traits of a given horticultural item at each stage from the farm to the market. We developed a procedure to extract and amplify by PCR DNA obtained from complex matrixes, such as dried figs and fig jam. Few fragmented DNA molecules can be recovered from food products. However, we were able to increase the yield of PCR reactions by successfully applying an enzymatic repair protocol to retrieved DNA.

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页码：546 / 549

页数：4

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