Magnolol inhibits leukotriene synthesis in rat basophilic leukemia-2H3 cells

被引：35

作者：

Hamasaki, Y ^{[1
]}

Kobayashi, I ^{[1
]}

Zaitu, M ^{[1
]}

Tsuji, K ^{[1
]}

Kita, M ^{[1
]}

Hayasaki, R ^{[1
]}

Muro, E ^{[1
]}

Yamamoto, S ^{[1
]}

Matsuo, M ^{[1
]}

Ichimaru, T ^{[1
]}

Miyazaki, S ^{[1
]}

机构：

[1] Saga Med Sch, Dept Pediat, Saga 8498501, Japan

来源：

PLANTA MEDICA | 1999年 / 65卷 / 03期

关键词：

magnolol; leukotriene (LT); rat basophilic leukemia-2H3 cell (RBL-2H3); cytosolic phospholipase A(2) (PLA(2)); 5-lipoxygenase (5-LO); Magnolia obovata Thunberg;

D O I：

10.1055/s-1999-13984

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

We have observed an inhibitory action of magnolol on the production of leukotriene (LT) C-4 and LTB4, important lipid mediators in allergy and inflammation. IgE- and A23187-stimulated production of LTC4 and LTB4 was measured by radioimmunoassay (RIA) in the absence or presence of various concentrations of magnolol in intact: rat basophilic leukemia (RBL)-2H3 cells. Magnolol dose-dependently inhibited synthesis of LTC4 and LTB4. Magnolol inhibited the IgE-mediated increase of; intracellular calcium ion concentration, resulting in the inhibition of cytosolic phospholipase A(2) (cPLA(2)) and possibly 5-lipoxygenase (5-LO), both calcium ion-dependent enzymes. In cell-free studies magnolol inhibited LTC,synthase activity LTA(4) hydrolase activity was only inhibited at the higher concentration (2.5 x 10(-5) M). These results indicate that magnolol inhibits production of LTs by inhibiting PLA(2), 5-LO, LTC4 synthase and LTA(4) hydrolase which are essential for LT-synthesis. Magnolol may have anti-allergic effect by blocking LT-synthesis.

引用

页码：222 / 226

页数：5

共 18 条

[1] LEUKOTRIENES ARE POTENT CONSTRICTORS OF HUMAN BRONCHI [J].