Effects of grinding surgical tissue specimens and smear staining methods on Buruli ulcer microscopic diagnosis

被引：10

作者：

Affolabi, Dissou ^{[1
,2
]}

Bankole, Honore

Ablordey, Anthony ^{[2
,3
]}

Hounnouga, Julie

Koutchakpo, Pierre

Sopoh, Ghislain

Aguiar, Julia

Dossou, Ange

Johnson, Roch Christian ^{[4
]}

Anagonou, Severin ^{[1
]}

Portaels, Francoise ^{[2
]}

机构：

[1] Lab Reference Mycobacteries, Cotonou, Benin

[2] Inst Trop Med, Mycobacteriol Unit, B-2000 Antwerp, Belgium

[3] Noguchi Mem Inst Med Res, Legon, Ghana

[4] Programme Natl Lutte Ulcere Buruli, Cotonou, Benin

来源：

TROPICAL MEDICINE & INTERNATIONAL HEALTH | 2008年 / 13卷 / 02期

关键词：

Buruli ulcer; smear; staining; tissue;

D O I：

10.1111/j.1365-3156.2007.01989.x

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

To optimize Buruli ulcer (BU) microscopic diagnosis, we compared two smear preparation methods from tissue specimens: smears made with tissue suspension after grinding and smears made directly with unground tissue. We also compared two smear staining methods: auramine and Ziehl-Neelsen (ZN). IS 2404-PCR was used as reference method. One hundred and thirty-one surgical tissue specimens from patients suspected of having BU were analyzed. Both smear preparation methods and both staining methods were equivalent in any combination. Thus we recommend ZN stained smears of unground tissue for peripheral treatment centres.

引用

页码：187 / 190

页数：4

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Yip, Marcus J. ;

Porter, Jessica L. ;

Fyfe, Janet A. M. ;

Lavender, Caroline J. ;

Portaels, Francoise ;

Rhodes, Martha ;

Kator, Howard ;

Colorni, Angelo ;

Jenkin, Grant A. ;

Stinear, Tim .

JOURNAL OF BACTERIOLOGY, 2007, 189 (05) :2021-2029

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