Transgenic tobacco expressing Pinellia ternata agglutinin confers enhanced resistance to aphids

被引：97

作者：

Yao, JH ^{[1
]}

Pang, YZ ^{[1
]}

Qi, HX ^{[1
]}

Wan, BL ^{[1
]}

Zhao, XY ^{[1
]}

Kong, WW ^{[1
]}

Sun, XF ^{[1
]}

Tang, KX ^{[1
]}

机构：

[1] Hubei Acad Agr Sci, Wuhan 430064, Hubei, Peoples R China

来源：

TRANSGENIC RESEARCH | 2003年 / 12卷 / 06期

关键词：

insect bioassay; peach potato aphid (Myzus persicae Sulzer); Pinellia ternata agglutinin (PTA); transformation; transgenic tobacco;

D O I：

10.1023/B:TRAG.0000005146.05655.7d

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Tobacco leaf discs were transformed with a plasmid, pBIPTA, containing the selectable marker neomycin phosphotransferase gene (nptII) and Pinellia ternata agglutinin gene (pta) via Agrobacterium tumefaciens- mediated transformation. Thirty-two independent transgenic tobacco plants were regenerated. PCR and Southern blot analyses confirmed that the pta gene had integrated into the plant genome and northern blot analysis revealed transgene expression at various levels in transgenic plants. Genetic analysis confirmed Mendelian segregation of the transgene in T1 progeny. Insect bioassays showed that transgenic plants expressing PTA inhibited significantly the growth of peach potato aphid (Myzus persicae Sulzer). This is the first report that transgenic plants expressing pta confer enhanced resistance to aphids. Our study indicates that the pta gene can be used as a supplement to the snowdrop ( Galanthus nivalis) lectin gene (gna) in the control of aphids, a sap-sucking insect pest causing significant yield losses of crops.

引用

页码：715 / 722

页数：8

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