A new Bauhinia monandra galactose-specific lectin purified in milligram quantities from secondary roots with antifungal and termiticidal activities

被引：33

作者：

Souza, Jayra D. ^{[1
]}

Silva, Maria B. R. ^{[1
]}

Argolo, Adriana C. C. ^{[1
]}

Napoleao, Thiago H. ^{[1
]}

Sa, Roberto A. ^{[2
]}

Correia, Maria T. S. ^{[1
]}

Paiva, Patricia M. G. ^{[1
]}

Silva, Michele D. C. ^{[3
]}

Coelho, Luana C. B. B. ^{[1
]}

机构：

[1] Univ Fed Pernambuco, Dept Bioquim, Ctr Ciencias Biol, BR-50670420 Recife, PE, Brazil

[2] Univ Fed Pernambuco, Ctr Acad Agreste, BR-55002970 Caruaru, PE, Brazil

[3] Univ Fed Rural Semi Arido, Dept Ciencias Anim, Mossoro, RN, Brazil

来源：

INTERNATIONAL BIODETERIORATION & BIODEGRADATION | 2011年 / 65卷 / 05期

关键词：

Bauhinia monandra; Root lectin; Antifungal activity; Termiticidal activity; NASUTITERMES-CORNIGER ISOPTERA; CARBOHYDRATE-RECOGNITION; MYRACRODRUON-URUNDEUVA; FUSARIUM-SOLANI; PURIFICATION; ELECTROPHORESIS; TERMITIDAE; RESISTANCE; PROTEINS; CLEAVAGE;

D O I：

10.1016/j.ibiod.2011.02.009

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

This work describes the purification in milligram quantities of a lectin from Bauhinia monandra secondary roots (BmoRoL) and its antifungal and termiticidal activities. The BmoRoL (6.2 mg) was isolated through ammonium sulfate fractionation and affinity chromatography on guar gel. Native lectin was resolved as a single band on polyacrylamide gel electrophoresis for basic proteins. Under denaturing and reducing conditions it appeared as a unique glycosylated polypeptide of 26 kDa. The highest agglutination activity of BmoRoL was found with glutaraldehyde-treated rabbit erythrocytes. BmoRoL showed antifungal activity against phytopathogenic species of Fusarium and was more active on Fusarium solani. The lectin also showed termiticidal activity on Nasutitermes corniger workers and soldiers with LC50 of 0.09 and 0.395 mg ml(-1) for 12 days. In conclusion, BmoRoL is a new antifungal and termiticidal lectin that can be purified in milligram quantities and has potential biotechnological application for control of agricultural pests. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：696 / 702

页数：7

共 65 条

[1]

Agrios G. N., 2005, PLANT PATHOL, P343

[2]

Purification and characterization of a lectin from the white shrimp Litopenaeus setiferus (Crustacea decapoda) hemolymph [J].

Alpuche, J ;

Pereyra, A ;

Agundis, C ;

Rosas, C ;

Pascual, C ;

Slomianny, MC ;

Vázquez, L ;

Zenteno, E .

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2005, 1724 (1-2) :86-93

[3]

Biodeteriorating agents associated with three tropical timber species [J].

Apetorgbor, MM ;

Darkwa, NA ;

Frimpong, O ;

Agyeman, VK .

FOREST ECOLOGY AND MANAGEMENT, 2004, 195 (03) :311-323

[4]

Purification and molecular characterization of a sialic acid specific lectin from the phytopathogenic fungus Macrophomina phaseolina [J].

Bhowal, J ;

Guha, AK ;

Chatterjee, BP .

CARBOHYDRATE RESEARCH, 2005, 340 (12) :1973-1982

[5]

BING DH, 1967, P SOC EXP BIOL MED, V124, P1166

[6]

Protecting wood from mould, decay, and termites with multi-component biocide systems [J].

Clausen, Carol A. ;

Yang, Vina .

INTERNATIONAL BIODETERIORATION & BIODEGRADATION, 2007, 59 (01) :20-24

[7]

Effect of Moringa oleifera lectin on development and mortality of Aedes aegypti larvae [J].

Coelho, Juliene S. ;

Santos, Nataly D. L. ;

Napoleao, Thiago H. ;

Gomes, Francis S. ;

Ferreira, Rodrigo S. ;

Zingali, Russolina B. ;

Coelho, Luana C. B. B. ;

Leite, Sonia P. ;

Navarro, Daniela M. A. F. ;

Paiva, Patricia M. G. .

CHEMOSPHERE, 2009, 77 (07) :934-938

[8]

COELHO LCB, 2000, PHYTOCHEM ANALYSIS, V11, P1

[9]

Purification of a glucose mannose specific lectin, isoform 1, from seeds of Cratylia mollis Mart (Camaratu bean) [J].

Correia, MTS ;

Coelho, LCBB .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1995, 55 (03) :261-273

[10]

Correia MTS, 2008, Recent Trends in Toxicology, P47

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