Production of glucuronan oligosaccharides using a new glucuronan lyase activity from a Trichoderma sp strain

被引：39

作者：

Delattre, C ^{[1
]}

Michaud, P ^{[1
]}

Lion, JM ^{[1
]}

Courtois, B ^{[1
]}

Courtois, J ^{[1
]}

机构：

[1] UPJV, IUT GB, Lab Glucides, EPMV,CNRS,FRE 2779, F-80025 Amiens, France

来源：

JOURNAL OF BIOTECHNOLOGY | 2005年 / 118卷 / 04期

关键词：

glucuronan; oligosaccharides; polysaccharide lyases;

D O I：

10.1016/j.jbiotec.2005.04.014

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

Sinorhizobium meliloti M5N1CS synthesizes a homopolymer of glucuronic acids beta-(1,4) linked and variably C-2 and/or C-3 O-acetylated. To obtain beta-Delta-(4,5)-unsaturated oligoglucuronans, various acetylated forms of this bacterial polymer were cleaved by a Trichoderma sp. GL2 glucuronan lyase. Oligomers with polymerization degrees up to 8 were then produced, purified by liquid chromatography (size exclusion and anions exchange) and characterized using H-1 NMR and ESI-Q/TOF-MS. Finally, the production (in gram quantity) of pure unsaturated oligoglucuronans non-acetylated (di- and trisaccharide) was investigated thanks to the complete depolymerization of deacetylated glucuronan. (C) 2005 Elsevier B.V. All rights reserved.

引用

页码：448 / 457

页数：10

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