Advances in capillary electrophoretically mediated microanalysis

被引：58

作者：

Van Dyck, S ^{[1
]}

Kaale, E ^{[1
]}

Nováková, S ^{[1
]}

Glatz, Z ^{[1
]}

Hoogmartens, J ^{[1
]}

Van Schepdael, A ^{[1
]}

机构：

[1] Katholieke Univ Leuven, Lab Farmaceut Chem Analyse Geneesmiddelen, Fac Farmaceut Wetenschappan, B-3000 Leuven, Belgium

来源：

ELECTROPHORESIS | 2003年 / 24卷 / 22-23期

关键词：

capillary electrophoresis; derivatization; electrophoretically mediated microanalysis; enzyme; review;

D O I：

10.1002/elps.200305636

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent developments in the use of capillary electrophoretic techniques for the in-line study of enzyme reactions and derivatization protocols are reviewed. The article is divided into two parts: (i) in-line enzyme reactions and (ii) in-line derivatization. The first part introduces electrophoretically mediated microanalysis (EMMA) and discusses and illustrates the different modes of EMMA. A literature overview is provided, starting from 1996, and the investigated enzymes are classified into two tables based on the mode of engagement (i.e., continuous or transient) of the developed EMMA-based assay. The second part starts with an introduction of the procedures and the nomenclature used in the area of in-line derivatization protocols based on EMMA. Reported derivatization procedures are discussed and classified in tables, according to the functional group that is derivatized.

引用

页码：3868 / 3878

页数：11

共 65 条

[41] In-line coupling of the enzymatic degradation of oligonucleotides with capillary polymer sieving electrophoresis [J].