Asymmetry in 13C-13C COSY spectra provides information on ligand geometry in paramagnetic proteins

被引：17

作者：

Bertini, I

Jiménez, B

Piccioli, M

Poggi, L

机构：

[1] Univ Florence, Magnet Resonance Ctr, I-50019 Florence, Italy

[2] Ecole Normale Super, Dept Chem, F-75005 Paris, France

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2005年 / 127卷 / 35期

关键词：

D O I：

10.1021/ja051058m

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The relative intensity of Cα-C- cross-peaks in homonuclear 13C COSY spectra depends on the relaxation properties of Cα and C- spins, which, in the proximity of a paramagnetic center, are related to the metal-to-carbon distance. Their quantitative analysis has lead, for the cerium-substituted dicalcium protein, calbindin D9k, to the straightforward identification of peaks arising from metal-coordinating groups. The monodentate or bidentate metal binding mode of carboxylates was identified directly via NMR. Copyright © 2005 American Chemical Society.

引用

收藏

页码：12216 / 12217

页数：2

相关论文

共 16 条

[1] A strategy for the NMR characterization of type II copper(II) proteins:: the case of the copper trafficking protein CopC from Pseudomonas syringae [J].

Arnesano, F ;

Banci, L ;

Bertini, I ;

Felli, IC ;

Luchinat, C ;

Thompsett, AR .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (24) :7200-7208

[2] The Protein Data Bank [J].

Berman, HM ;

Westbrook, J ;

Feng, Z ;

Gilliland, G ;

Bhat, TN ;

Weissig, H ;

Shindyalov, IN ;

Bourne, PE .

NUCLEIC ACIDS RESEARCH, 2000, 28 (01) :235-242

[3] Paramagnetism-based versus classical constraints:: An analysis of the solution structure of Ca Ln calbindin D9k [J].

Bertini, I ;

Donaire, A ;

Jiménez, B ;

Luchinat, C ;

Parigi, G ;

Piccioli, M ;

Poggi, L .

JOURNAL OF BIOMOLECULAR NMR, 2001, 21 (02) :85-98

[4] Paramagnetic probes in metalloproteins [J].

Bertini, I ;

Luchinat, C ;

Piccioli, M .

NUCLEAR MAGNETIC RESONANCE OF BIOLOGICAL MACROMOLECULES, PT B, 2001, 339 :314-340

[5]

Bertini I, 2001, CHEMBIOCHEM, V2, P550, DOI 10.1002/1439-7633(20010803)2:7/8<550::AID-CBIC550>3.3.CO

[6]

2-K

[7]

BERTINI I, 1994, CONCEPT MAGNETIC RES, V6, P307

[8] DIGITAL FILTERING WITH A SINUSOIDAL WINDOW FUNCTION - ALTERNATIVE TECHNIQUE FOR RESOLUTION ENHANCEMENT IN FT NMR [J].

DEMARCO, A ;

WUTHRICH, K .

JOURNAL OF MAGNETIC RESONANCE, 1976, 24 (02) :201-204

[9] Experimental characterization of models for backbone picosecond dynamics in proteins. Quantification of NMR auto- and cross-correlation relaxation mechanisms involving different nuclei of the peptide plane [J].

Fischer, MWF ;

Zeng, L ;

Pang, YX ;

Hu, WD ;

Majumdar, A ;

Zuiderweg, ERP .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1997, 119 (51) :12629-12642

[10] SYMMETRY IN COHERENCE TRANSFER APPLICATION TO TWO-DIMENSIONAL NMR [J].

GRIESINGER, C ;

GEMPERLE, C ;

SORENSEN, OW ;

ERNST, RR .

MOLECULAR PHYSICS, 1987, 62 (02) :295-332