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Identification of NPR1-dependent and independent genes early induced by salicylic acid treatment in Arabidopsis
被引:76
作者:
Blanco, F
Garretón, V
Frey, N
Dominguez, C
Pérez-Acle, T
Van Der Straeten, D
Jordana, X
Holuigue, L
机构:
[1] Pontificia Univ Catolica Chile, Fac Ciencias Biol, Dept Mol Genet & Microbiol, Santiago, Chile
[2] Pontificia Univ Catolica Chile, Fac Ciencias Biol, Ctr Genom & Bioinformat, Santiago, Chile
[3] Univ Ghent, Dept Mol Genet, Unit Plant Hormone Signaling & Bioimaging, B-9000 Ghent, Belgium
[4] AustralBiotech SA, Santiago, Chile
关键词:
as-1;
cDNA-AFLP;
cell protection to stress;
early activated genes;
NPR1;
salicylic acid;
D O I:
10.1007/s11103-005-2227-x
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
Salicylic acid (SA) plays a crucial role in stress resistance in plants by modifying the expression of a battery of genes. In this paper, we report the identification of a group of early SA-regulated genes of Arabidopsis (activated between 0.5-2.5 h), using the cDNA-amplified fragment length polymorphism technique (cDNA-AFLP). Using 128 different primer combinations, we identified several genes based on their differential expression during SA treatment. Among these, we identified 12 genes up-regulated by SA whose patterns of induction were confirmed by Northern analysis. The identified genes can be grouped into two functional groups: Group 1: genes involved in cell protection (i.e. glycosyltransferases, glutathion S-transferases), and Group 2: genes involved in signal transduction (protein kinases and transcription factors). We also evaluated NPR1 requirement for the induction of the 12 up-regulated genes, and found that only those belonging to Group 2 require this co-activator for their expression. In silico analysis of the promoter sequences of the up-regulated genes, allowed us to identify putative cis-elements over-represented in these genes. Interestingly, as-1-like elements, previously characterized as SA-responsive elements, were specifically over-represented in Group 1 genes. The identification of early SA-regulated genes is an important step towards understanding the complex role of this hormone in plant stress resistance.
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页码:927 / 944
页数:18
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