Identification of NPR1-dependent and independent genes early induced by salicylic acid treatment in Arabidopsis

被引:76
作者
Blanco, F
Garretón, V
Frey, N
Dominguez, C
Pérez-Acle, T
Van Der Straeten, D
Jordana, X
Holuigue, L
机构
[1] Pontificia Univ Catolica Chile, Fac Ciencias Biol, Dept Mol Genet & Microbiol, Santiago, Chile
[2] Pontificia Univ Catolica Chile, Fac Ciencias Biol, Ctr Genom & Bioinformat, Santiago, Chile
[3] Univ Ghent, Dept Mol Genet, Unit Plant Hormone Signaling & Bioimaging, B-9000 Ghent, Belgium
[4] AustralBiotech SA, Santiago, Chile
关键词
as-1; cDNA-AFLP; cell protection to stress; early activated genes; NPR1; salicylic acid;
D O I
10.1007/s11103-005-2227-x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Salicylic acid (SA) plays a crucial role in stress resistance in plants by modifying the expression of a battery of genes. In this paper, we report the identification of a group of early SA-regulated genes of Arabidopsis (activated between 0.5-2.5 h), using the cDNA-amplified fragment length polymorphism technique (cDNA-AFLP). Using 128 different primer combinations, we identified several genes based on their differential expression during SA treatment. Among these, we identified 12 genes up-regulated by SA whose patterns of induction were confirmed by Northern analysis. The identified genes can be grouped into two functional groups: Group 1: genes involved in cell protection (i.e. glycosyltransferases, glutathion S-transferases), and Group 2: genes involved in signal transduction (protein kinases and transcription factors). We also evaluated NPR1 requirement for the induction of the 12 up-regulated genes, and found that only those belonging to Group 2 require this co-activator for their expression. In silico analysis of the promoter sequences of the up-regulated genes, allowed us to identify putative cis-elements over-represented in these genes. Interestingly, as-1-like elements, previously characterized as SA-responsive elements, were specifically over-represented in Group 1 genes. The identification of early SA-regulated genes is an important step towards understanding the complex role of this hormone in plant stress resistance.
引用
收藏
页码:927 / 944
页数:18
相关论文
共 72 条
[71]   Knockout analysis of Arabidopsis transcription factors TGA2, TGA5, and TGA6 reveals their redundant and essential roles in systemic acquired resistance [J].
Zhang, YL ;
Tessaro, MJ ;
Lassner, M ;
Li, X .
PLANT CELL, 2003, 15 (11) :2647-2653
[72]   Interaction of NPR1 with basic leucine zipper protein transcription factors that bind sequences required for salicylic acid induction of the PR-1 gene [J].
Zhang, YL ;
Fan, WH ;
Kinkema, M ;
Li, X ;
Dong, XN .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (11) :6523-6528