CRISPR-Cas9 enables conditional mutagenesis of challenging loci

被引：9

作者：

Schick, Joel A. ^{[1
,7
]}

Seisenberger, Claudia ^{[1
]}

Beig, Joachim ^{[1
]}

Buerger, Antje ^{[1
]}

Iyer, Vivek ^{[2
]}

Maier, Viola ^{[1
]}

Perera, Sajith ^{[2
]}

Rosen, Barry ^{[2
,3
]}

Skarnes, William C. ^{[2
]}

Wurst, Wolfgang ^{[1
,4
,5
,6
]}

机构：

[1] Helmholtz Zentrum Munchen, Inst Dev Genet, Ingolstadter Landstr 1, D-85764 Neuherberg, Germany

[2] Wellcome Trust Sanger Inst, Wellcome Trust Genome Campus, Cambridge, England

[3] AstraZeneca, Darwin Bldg,Unit 310,Cambridge Sci Pk,Milton Rd, Cambridge CB4 0WG, England

[4] Tech Univ Munchen eihenstepha, Helmholtz Zentrum Munchen, Ingolstadter Landstr 1, D-85764 Neuherberg, Germany

[5] German Ctr Neurodegenerat Dis DZNE, Site Munich,Feodor Lynen Str 17, D-81377 Munich, Germany

[6] Univ Munich, Munich Cluster Syst Neurol SyNergy, Adolf Butenandt Inst, Feodor Lynen Str 17, D-81377 Munich, Germany

[7] Helmholtz Zentrum Munchen, Inst Mol Toxicol & Pharmacol, Ingolstadter Landstr 1, D-85764 Neuherberg, Germany

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

关键词：

ONE-STEP GENERATION; HOMOLOGOUS RECOMBINATION; GENOME; CAS9; RESOURCE; GENES;

D O I：

10.1038/srep32326

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

The International Knockout Mouse Consortium (IKMC) has produced a genome-wide collection of 15,000 isogenic targeting vectors for conditional mutagenesis in C57BL/6N mice. Although most of the vectors have been used successfully in murine embryonic stem (ES) cells, there remain a set of nearly two thousand genes that have failed to target even after several attempts. Recent attention has turned to the use of new genome editing technology for the generation of mutant alleles in mice. Here, we demonstrate how Cas9-assisted targeting can be combined with the IKMC targeting vector resource to generate conditional alleles in genes that have previously eluded targeting using conventional methods.

引用

页数：6

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