Effect of renal dialysis therapy modality on T cell cytokine production

被引:46
作者
Zamauskaite, A
Perez-Cruz, I
Yaqoob, MM
Madrigal, JA
Cohen, SBA
机构
[1] Royal Free Hosp, Anthony Nolan Bone Marrow Trust, London NW3 2QG, England
[2] Royal Free Hosp, Dept Haematol, London NW3 2QG, England
[3] St Bartholomews Hosp, Renal Unit, London, England
关键词
dialysis; Th1; Th2; intracellular cytokines;
D O I
10.1093/ndt/14.1.49
中图分类号
R3 [基础医学]; R4 [临床医学];
学科分类号
1001 ; 1002 ; 100602 ;
摘要
Introduction. Dialysis has been associated with acute changes in the complement activation status, granulocyte markers, macrophage function, T cell activation and the release of pro-inflammatory cytokines. The most common analysis of cytokine production in patients on dialysis has focused on the changes in monokines (particularly IL-1 and TNF alpha), however it is becoming clear that T cell cytokines play a major role in the impaired lymphocyte function of dialysis patients. Methods. To assess the effect of dialysis modality on T cell function we analysed the ability of T cells within peripheral blood mononuclear cell populations (PBMC) to produce cytokines after mitogen (phorbol-12-myristate-13-acetate; PMA and Ionomycin; I) stimulation in patients on peritoneal dialysis (PD) compared to low flux haemodialysis (HD) and normal individuals (controls). Results. In control PBMC, PMA+I stimulation significantly increased the percentage of CD3(+) cells expressing IL-2, IFN gamma, TNF alpha, IL-4 and IL-10, as expected. However, although mitogen stimulation significantly enhanced the percentage of the classical Th1 cytokines (IL-2, IFN gamma and TNF alpha) in the low flux HD PBMC, it had no effect on CD3(+) IL-2 or CD3(+) TNF alpha producing cells in the PD group. In contrast, the percentage of T cells producing Th2 cytokines (IL-4 and IL-10) could not be consistently enhanced by mitogen in either dialysis group. Conclusions. We suggest that PD alters the ability of T cells to produce cytokines, possibly by causing an 'exhaustion' of the Th1 cells, thereby preventing cells to produce cytokine on ex vivo stimulation Furthermore, since T cells from both low flux HD and PD groups could not be induced to produce Th2 cytokines we suggest that uraemia or dialysis per se inhibits T cells from producing Th2 cytokines.
引用
收藏
页码:49 / 55
页数:7
相关论文
共 41 条
[21]   INFLUENCE OF 1ST AND LONG-TERM DIALYSIS ON UREMIA-ASSOCIATED INCREASED BASAL PRODUCTION OF INTERLEUKIN-1 AND TUMOR-NECROSIS-FACTOR-ALPHA BY CIRCULATING MONOCYTES [J].
HERBELIN, A ;
URENA, P ;
NGUYEN, AT ;
ZINGRAFF, J ;
DESCAMPSLATSCHA, B .
NEPHROLOGY DIALYSIS TRANSPLANTATION, 1991, 6 (05) :349-357
[22]   MODULATION OF GRANULOCYTE LAM-1 AND MAC-1 DURING DIALYSIS - A PROSPECTIVE, RANDOMIZED CONTROLLED TRIAL [J].
HIMMELFARB, J ;
ZAOUI, P ;
HAKIM, R .
KIDNEY INTERNATIONAL, 1992, 41 (02) :388-395
[23]  
JORRES A, 1992, INT J ARTIF ORGANS, V15, P79
[24]   ARE CD4(+) T(H)1 CELLS PRO-INFLAMMATORY OR ANTIINFLAMMATORY - THE RATIO OF IL-10 TO IFN-GAMMA OR IL-2 DETERMINES THEIR FUNCTION [J].
KATSIKIS, PD ;
COHEN, SBA ;
LONDEI, M ;
FELDMANN, M .
INTERNATIONAL IMMUNOLOGY, 1995, 7 (08) :1287-1294
[25]  
Lin Y, 1993, AM J NEPHROL, V16, P293
[26]  
LONNEMANN G, 1987, LYMPHOKINE RES, V6, P63
[27]  
LONNEMANN G, 1988, J LAB CLIN MED, V112, P76
[28]   The expanding universe of T-cell subsets: Th1, Th2 and more [J].
Mosmann, TR ;
Sad, S .
IMMUNOLOGY TODAY, 1996, 17 (03) :138-146
[29]  
MOSMANN TR, 1986, J IMMUNOL, V136, P2348
[30]   COMPARISON OF 1ST USE AND REUSE OF CUPROPHAN MEMBRANES ON INTERLEUKIN-1 RECEPTOR ANTAGONIST AND INTERLEUKIN-1-BETA PRODUCTION BY BLOOD MONONUCLEAR-CELLS [J].
PEREIRA, BJG ;
KING, AJ ;
POUTSIAKA, DD ;
STROM, JA ;
DINARELLO, CA .
AMERICAN JOURNAL OF KIDNEY DISEASES, 1993, 22 (02) :288-295