Application of dried blood spot specimens for serologic subtyping of human immunodeficiency virus type 1 in Thailand

被引：13

作者：

Chanbancherd, P

Brown, AE

Trichavaroj, R

Tienamporn, P

Puthakird, P

Limpairojn, N

VanCott, TC

De Souza, MS ^{[1
]}

机构：

[1] Henry M Jackson Fdn Advancement Mil Med, AFRIMS, Bangkok 10400, Thailand

[2] Armed Inst Pathol, Bangkok, Thailand

[3] Armed Forces Res Inst Med Sci, Bangkok 10400, Thailand

[4] Henry M Jackson Fdn Advancement Mil Med, Rockville, MD USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1999年 / 37卷 / 03期

关键词：

D O I：

10.1128/JCM.37.3.804-806.1999

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Dried blood spot (DBS) specimens were assessed as an alternative to plasma for human immunodeficiency virus type 1 (HIV-1) serotyping by V3 loop peptide enzyme immunoassay. Nested PCR capable of distinguishing HIV-1 subtypes B and E was used as the reference standard. Ninety-two percent of DBS samples were typeable as either HIV-1 subtype B or E. Serotype results with DBS and plasm;! were identical for 254 of 257 specimens. A simple DBS collection method provides a convenient alternative for conducting HIV-1 serotype pe surveillance while retaining sensitivity and specificity.

引用

页码：804 / 806

页数：3

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